SRX18700902 GSM6833440_r1 SRP413083 PRJNA912209 SRS16141977 GSM6833440 GSM6833440 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700903 GSM6833441_r1 SRP413083 PRJNA912209 SRS16141978 GSM6833441 GSM6833441 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700904 GSM6833442_r1 SRP413083 PRJNA912209 SRS16141979 GSM6833442 GSM6833442 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700905 GSM6833443_r1 SRP413083 PRJNA912209 SRS16141980 GSM6833443 GSM6833443 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700906 GSM6833444_r1 SRP413083 PRJNA912209 SRS16141981 GSM6833444 GSM6833444 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700907 GSM6833445_r1 SRP413083 PRJNA912209 SRS16141982 GSM6833445 GSM6833445 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700908 GSM6833446_r1 SRP413083 PRJNA912209 SRS16141983 GSM6833446 GSM6833446 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700909 GSM6833447_r1 SRP413083 PRJNA912209 SRS16141984 GSM6833447 GSM6833447 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700910 GSM6833448_r1 SRP413083 PRJNA912209 SRS16141985 GSM6833448 GSM6833448 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700911 GSM6833449_r1 SRP413083 PRJNA912209 SRS16141986 GSM6833449 GSM6833449 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700912 GSM6833450_r1 SRP413083 PRJNA912209 SRS16141987 GSM6833450 GSM6833450 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700913 GSM6833451_r1 SRP413083 PRJNA912209 SRS16141988 GSM6833451 GSM6833451 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700914 GSM6833452_r1 SRP413083 PRJNA912209 SRS16141989 GSM6833452 GSM6833452 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700915 GSM6833453_r1 SRP413083 PRJNA912209 SRS16141990 GSM6833453 GSM6833453 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700916 GSM6833454_r1 SRP413083 PRJNA912209 SRS16141991 GSM6833454 GSM6833454 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700917 GSM6833455_r1 SRP413083 PRJNA912209 SRS16141992 GSM6833455 GSM6833455 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700918 GSM6833456_r1 SRP413083 PRJNA912209 SRS16141993 GSM6833456 GSM6833456 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700919 GSM6833457_r1 SRP413083 PRJNA912209 SRS16141994 GSM6833457 GSM6833457 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700920 GSM6833458_r1 SRP413083 PRJNA912209 SRS16141995 GSM6833458 GSM6833458 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700921 GSM6833459_r1 SRP413083 PRJNA912209 SRS16141997 GSM6833459 GSM6833459 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700922 GSM6833460_r1 SRP413083 PRJNA912209 SRS16141996 GSM6833460 GSM6833460 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700923 GSM6833461_r1 SRP413083 PRJNA912209 SRS16141998 GSM6833461 GSM6833461 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700924 GSM6833462_r1 SRP413083 PRJNA912209 SRS16141999 GSM6833462 GSM6833462 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700925 GSM6833463_r1 SRP413083 PRJNA912209 SRS16142000 GSM6833463 GSM6833463 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700926 GSM6833464_r1 SRP413083 PRJNA912209 SRS16142001 GSM6833464 GSM6833464 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700927 GSM6833465_r1 SRP413083 PRJNA912209 SRS16142002 GSM6833465 GSM6833465 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700928 GSM6833466_r1 SRP413083 PRJNA912209 SRS16142003 GSM6833466 GSM6833466 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700929 GSM6833467_r1 SRP413083 PRJNA912209 SRS16142004 GSM6833467 GSM6833467 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700930 GSM6833468_r1 SRP413083 PRJNA912209 SRS16142005 GSM6833468 GSM6833468 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700931 GSM6833469_r1 SRP413083 PRJNA912209 SRS16142006 GSM6833469 GSM6833469 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700932 GSM6833470_r1 SRP413083 PRJNA912209 SRS16142007 GSM6833470 GSM6833470 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700933 GSM6833471_r1 SRP413083 PRJNA912209 SRS16142008 GSM6833471 GSM6833471 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700934 GSM6833472_r1 SRP413083 PRJNA912209 SRS16142009 GSM6833472 GSM6833472 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700935 GSM6833473_r1 SRP413083 PRJNA912209 SRS16142010 GSM6833473 GSM6833473 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700936 GSM6833474_r1 SRP413083 PRJNA912209 SRS16142011 GSM6833474 GSM6833474 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700937 GSM6833475_r1 SRP413083 PRJNA912209 SRS16142012 GSM6833475 GSM6833475 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700938 GSM6833476_r1 SRP413083 PRJNA912209 SRS16142013 GSM6833476 GSM6833476 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550 SRX18700939 GSM6833477_r1 SRP413083 PRJNA912209 SRS16142014 GSM6833477 GSM6833477 OTHER GENOMIC other Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers. NextSeq 550