SRX18711453 GSM6840723_r1 SRP413233 PRJNA912647 SRS16150894 GSM6840723 GSM6840723 RNA-Seq TRANSCRIPTOMIC cDNA Two days after the immunization, donor B cells were magnetically enriched from pooled spleens of recipient mice using APC conjugated anti-CD45.1 (eBiosciences: Cat# 17-0453-82) and anti-APC MicroBeads (Miltenyi Biotec), with the MACS system (Miltenyi Biotec). After enrichment, cells were further stained with the respective Abs and CD45.1+ B220+ CD138- FSChi cells were sorted by using FACSAria III (BD Biosciences). Total RNA isolation was achieved using RNeasy Plus Micro Kit (QIAGEN). cDNA libraries were prepared with TaKaRa SmartSeq Stranded kit (Takara) following manufacturer's protocols. Illumina NovaSeq 6000 SRX18711454 GSM6840724_r1 SRP413233 PRJNA912647 SRS16150895 GSM6840724 GSM6840724 RNA-Seq TRANSCRIPTOMIC cDNA Two days after the immunization, donor B cells were magnetically enriched from pooled spleens of recipient mice using APC conjugated anti-CD45.1 (eBiosciences: Cat# 17-0453-82) and anti-APC MicroBeads (Miltenyi Biotec), with the MACS system (Miltenyi Biotec). After enrichment, cells were further stained with the respective Abs and CD45.1+ B220+ CD138- FSChi cells were sorted by using FACSAria III (BD Biosciences). Total RNA isolation was achieved using RNeasy Plus Micro Kit (QIAGEN). cDNA libraries were prepared with TaKaRa SmartSeq Stranded kit (Takara) following manufacturer's protocols. Illumina NovaSeq 6000 SRX18711455 GSM6840725_r1 SRP413233 PRJNA912647 SRS16150896 GSM6840725 GSM6840725 RNA-Seq TRANSCRIPTOMIC cDNA Two days after the immunization, donor B cells were magnetically enriched from pooled spleens of recipient mice using APC conjugated anti-CD45.1 (eBiosciences: Cat# 17-0453-82) and anti-APC MicroBeads (Miltenyi Biotec), with the MACS system (Miltenyi Biotec). After enrichment, cells were further stained with the respective Abs and CD45.1+ B220+ CD138- FSChi cells were sorted by using FACSAria III (BD Biosciences). Total RNA isolation was achieved using RNeasy Plus Micro Kit (QIAGEN). cDNA libraries were prepared with TaKaRa SmartSeq Stranded kit (Takara) following manufacturer's protocols. Illumina NovaSeq 6000 SRX18711456 GSM6840726_r1 SRP413233 PRJNA912647 SRS16150897 GSM6840726 GSM6840726 RNA-Seq TRANSCRIPTOMIC cDNA Two days after the immunization, donor B cells were magnetically enriched from pooled spleens of recipient mice using APC conjugated anti-CD45.1 (eBiosciences: Cat# 17-0453-82) and anti-APC MicroBeads (Miltenyi Biotec), with the MACS system (Miltenyi Biotec). After enrichment, cells were further stained with the respective Abs and CD45.1+ B220+ CD138- FSChi cells were sorted by using FACSAria III (BD Biosciences). Total RNA isolation was achieved using RNeasy Plus Micro Kit (QIAGEN). cDNA libraries were prepared with TaKaRa SmartSeq Stranded kit (Takara) following manufacturer's protocols. Illumina NovaSeq 6000