SRX19785818 GSM7118508_r1 SRP429553 GSE228300 SRS17148883 GSM7118508 GSM7118508 OTHER TRANSCRIPTOMIC other Frozen kidney biopsy specimens were obtained after appropriate patient consent and in accordance with Partners Healthcare IRB and institutional guidelines. After first removing the surrounding optimal cutting temperature (OCT) embedding medium with PBS, a previously published protocol using salt Tris-based buffers was used to isolate single nuclei. 8,000 single nuclei were then loaded into each channel of the Chromium single cell 3' chip (v3; 10x Genomics, Pleasanton, USA). Single nuclei were partitioned into gel bead-in-emulsions (GEMs) and incubated to generate barcoded cDNA by reverse transcription. Barcoded cDNA was then amplified by PCR prior to library construction. Fragmentation, sample index, and adaptor ligation, and PCR were used to generate libraries of paired-end constructs according to the manufacturer's recommendations (10x Genomics, Pleasanton, USA). Libraries were pooled and sequenced using the Illumina HiSeq X system (San Diego, USA). single nuclei RNA sequencing (snRNA seq) HiSeq X Ten SRX19785819 GSM7118509_r1 SRP429553 GSE228300 SRS17148885 GSM7118509 GSM7118509 OTHER TRANSCRIPTOMIC other Frozen kidney biopsy specimens were obtained after appropriate patient consent and in accordance with Partners Healthcare IRB and institutional guidelines. After first removing the surrounding optimal cutting temperature (OCT) embedding medium with PBS, a previously published protocol using salt Tris-based buffers was used to isolate single nuclei. 8,000 single nuclei were then loaded into each channel of the Chromium single cell 3' chip (v3; 10x Genomics, Pleasanton, USA). Single nuclei were partitioned into gel bead-in-emulsions (GEMs) and incubated to generate barcoded cDNA by reverse transcription. Barcoded cDNA was then amplified by PCR prior to library construction. Fragmentation, sample index, and adaptor ligation, and PCR were used to generate libraries of paired-end constructs according to the manufacturer's recommendations (10x Genomics, Pleasanton, USA). Libraries were pooled and sequenced using the Illumina HiSeq X system (San Diego, USA). single nuclei RNA sequencing (snRNA seq) HiSeq X Ten SRX19785820 GSM7118510_r1 SRP429553 GSE228300 SRS17148887 GSM7118510 GSM7118510 OTHER TRANSCRIPTOMIC other Frozen kidney biopsy specimens were obtained after appropriate patient consent and in accordance with Partners Healthcare IRB and institutional guidelines. After first removing the surrounding optimal cutting temperature (OCT) embedding medium with PBS, a previously published protocol using salt Tris-based buffers was used to isolate single nuclei. 8,000 single nuclei were then loaded into each channel of the Chromium single cell 3' chip (v3; 10x Genomics, Pleasanton, USA). Single nuclei were partitioned into gel bead-in-emulsions (GEMs) and incubated to generate barcoded cDNA by reverse transcription. Barcoded cDNA was then amplified by PCR prior to library construction. Fragmentation, sample index, and adaptor ligation, and PCR were used to generate libraries of paired-end constructs according to the manufacturer's recommendations (10x Genomics, Pleasanton, USA). Libraries were pooled and sequenced using the Illumina HiSeq X system (San Diego, USA). single nuclei RNA sequencing (snRNA seq) HiSeq X Ten