SRX19322484 GSM7036049_r1 SRP421692 PRJNA933163 SRS16720607 GSM7036049 GSM7036049 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Then Cells were added to each channel. After generation of GEMs, reverse transcription reactions were engaged barcoded full-length cDNA followed by the disruption of emulsions using the recovery agent and cDNA clean up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed, and index adaptor ligated and library amplification. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol. Illumina NovaSeq 6000 SRX19322485 GSM7036050_r1 SRP421692 PRJNA933163 SRS16720606 GSM7036050 GSM7036050 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Then Cells were added to each channel. After generation of GEMs, reverse transcription reactions were engaged barcoded full-length cDNA followed by the disruption of emulsions using the recovery agent and cDNA clean up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed, and index adaptor ligated and library amplification. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol. Illumina NovaSeq 6000