SRX19820065 GSM7124082_r1 SRP430136 PRJNA950373 SRS17180421 GSM7124082 GSM7124082 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820066 GSM7124083_r1 SRP430136 PRJNA950373 SRS17180420 GSM7124083 GSM7124083 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820067 GSM7124084_r1 SRP430136 PRJNA950373 SRS17180423 GSM7124084 GSM7124084 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820068 GSM7124085_r1 SRP430136 PRJNA950373 SRS17180422 GSM7124085 GSM7124085 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820069 GSM7124086_r1 SRP430136 PRJNA950373 SRS17180424 GSM7124086 GSM7124086 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820070 GSM7124087_r1 SRP430136 PRJNA950373 SRS17180425 GSM7124087 GSM7124087 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820071 GSM7124088_r1 SRP430136 PRJNA950373 SRS17180426 GSM7124088 GSM7124088 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820072 GSM7124089_r1 SRP430136 PRJNA950373 SRS17180427 GSM7124089 GSM7124089 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820073 GSM7124090_r1 SRP430136 PRJNA950373 SRS17180428 GSM7124090 GSM7124090 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820074 GSM7124091_r1 SRP430136 PRJNA950373 SRS17180429 GSM7124091 GSM7124091 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820075 GSM7124092_r1 SRP430136 PRJNA950373 SRS17180430 GSM7124092 GSM7124092 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000 SRX19820076 GSM7124093_r1 SRP430136 PRJNA950373 SRS17180431 GSM7124093 GSM7124093 OTHER TRANSCRIPTOMIC other Cells were flash frozen by submerging the bottoms of dishes in liquid nitrogen. Cells were collected and lysed by scraping in lysis buffer containing cycloheximide. Cell lysates were triturated and clarified by centrifugation. Input samples were extracted from aliquot of lysate using trizol reagent according to the manufacturer's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNAeasy column and purified according to kit instructions. For ribosome prototected fragments, lyates were treated with RNase I and loaded onto 15%–45% sucrose gradients. Samples were fractionated and fractions containing 80S monosomes were precipitated with ethanol. After precipitation, monosome containing fractions were resuspended in trizol reagent. Sample workup was performed with a Zymo direct-zol kit following the manufacturer's protocol. Samples were ribodepleted using Ribopoo ribosome RNA Homo sapiens depletion kit supplemented with custom-made oligos. RPF and input fractions were fragmented and size-selected on denaturing Urea PAGE gels. 3' adaptors sequencing adaptors were added with RNA ligase and reactions were reverse-transcribed with SuperScript IV. Sample cDNA was circularized with circLigase and libraries were PCR amplified to add Illumina sequencing primers. Libraries were sequenced on a NextSeq 2000 P2 instrument. NextSeq 2000