SRP435421 PRJNA964779 GSE231405 Single-cell transcriptome profiles of wild-type and IRF8KO microglia [scRNA-seq] The role of the lineage-determining transcription factor IRF8 in microglia remains unelucidated. We analyzed the transcriptome diversity of wild-type and IRF8KO microglia. Deep-sequencing and subsequent differential analysis revealed the significance of the IRF8-dependent transcription on microglial heterogeneity and self-renewing. This study provides a novel insight into understanding IRF8-mediated transcriptional programs in microglia. Overall design: Transcriptome profiles of adult WT and IRF8KO microglia were analyzed at a single-cell level using 10xGenomics. GSE231405 pubmed 37645844 parent_bioproject PRJNA964768