SRX19314649 GSM7034457_r1 SRP421523 PRJNA932800 SRS16713405 GSM7034457 GSM7034457 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314650 GSM7034458_r1 SRP421523 PRJNA932800 SRS16713407 GSM7034458 GSM7034458 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314651 GSM7034459_r1 SRP421523 PRJNA932800 SRS16713406 GSM7034459 GSM7034459 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314652 GSM7034460_r1 SRP421523 PRJNA932800 SRS16713408 GSM7034460 GSM7034460 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314653 GSM7034461_r1 SRP421523 PRJNA932800 SRS16713409 GSM7034461 GSM7034461 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314654 GSM7034462_r1 SRP421523 PRJNA932800 SRS16713410 GSM7034462 GSM7034462 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314655 GSM7034463_r1 SRP421523 PRJNA932800 SRS16713411 GSM7034463 GSM7034463 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314656 GSM7034464_r1 SRP421523 PRJNA932800 SRS16713412 GSM7034464 GSM7034464 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314657 GSM7034465_r1 SRP421523 PRJNA932800 SRS16713413 GSM7034465 GSM7034465 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314658 GSM7034466_r1 SRP421523 PRJNA932800 SRS16713414 GSM7034466 GSM7034466 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314659 GSM7034467_r1 SRP421523 PRJNA932800 SRS16713416 GSM7034467 GSM7034467 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314660 GSM7034468_r1 SRP421523 PRJNA932800 SRS16713415 GSM7034468 GSM7034468 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314661 GSM7034469_r1 SRP421523 PRJNA932800 SRS16713417 GSM7034469 GSM7034469 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314662 GSM7034470_r1 SRP421523 PRJNA932800 SRS16713418 GSM7034470 GSM7034470 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314663 GSM7034471_r1 SRP421523 PRJNA932800 SRS16713419 GSM7034471 GSM7034471 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314664 GSM7034472_r1 SRP421523 PRJNA932800 SRS16713421 GSM7034472 GSM7034472 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314665 GSM7034473_r1 SRP421523 PRJNA932800 SRS16713422 GSM7034473 GSM7034473 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314666 GSM7034474_r1 SRP421523 PRJNA932800 SRS16713420 GSM7034474 GSM7034474 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314667 GSM7034491_r1 SRP421523 PRJNA932800 SRS16713423 GSM7034491 GSM7034491 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314668 GSM7034492_r1 SRP421523 PRJNA932800 SRS16713424 GSM7034492 GSM7034492 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314669 GSM7034493_r1 SRP421523 PRJNA932800 SRS16713425 GSM7034493 GSM7034493 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314670 GSM7034494_r1 SRP421523 PRJNA932800 SRS16713426 GSM7034494 GSM7034494 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314671 GSM7034495_r1 SRP421523 PRJNA932800 SRS16713427 GSM7034495 GSM7034495 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314672 GSM7034496_r1 SRP421523 PRJNA932800 SRS16713428 GSM7034496 GSM7034496 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314673 GSM7034497_r1 SRP421523 PRJNA932800 SRS16713429 GSM7034497 GSM7034497 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314674 GSM7034498_r1 SRP421523 PRJNA932800 SRS16713431 GSM7034498 GSM7034498 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314675 GSM7034499_r1 SRP421523 PRJNA932800 SRS16713430 GSM7034499 GSM7034499 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314676 GSM7034500_r1 SRP421523 PRJNA932800 SRS16713432 GSM7034500 GSM7034500 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314677 GSM7034501_r1 SRP421523 PRJNA932800 SRS16713433 GSM7034501 GSM7034501 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314678 GSM7034502_r1 SRP421523 PRJNA932800 SRS16713434 GSM7034502 GSM7034502 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314679 GSM7034503_r1 SRP421523 PRJNA932800 SRS16713435 GSM7034503 GSM7034503 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314680 GSM7034504_r1 SRP421523 PRJNA932800 SRS16713436 GSM7034504 GSM7034504 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314681 GSM7034505_r1 SRP421523 PRJNA932800 SRS16713437 GSM7034505 GSM7034505 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314682 GSM7034506_r1 SRP421523 PRJNA932800 SRS16713439 GSM7034506 GSM7034506 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314683 GSM7034507_r1 SRP421523 PRJNA932800 SRS16713438 GSM7034507 GSM7034507 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314684 GSM7034508_r1 SRP421523 PRJNA932800 SRS16713440 GSM7034508 GSM7034508 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314685 GSM7034509_r1 SRP421523 PRJNA932800 SRS16713441 GSM7034509 GSM7034509 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314686 GSM7034510_r1 SRP421523 PRJNA932800 SRS16713442 GSM7034510 GSM7034510 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314687 GSM7034511_r1 SRP421523 PRJNA932800 SRS16713443 GSM7034511 GSM7034511 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314688 GSM7034512_r1 SRP421523 PRJNA932800 SRS16713444 GSM7034512 GSM7034512 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314689 GSM7034513_r1 SRP421523 PRJNA932800 SRS16713445 GSM7034513 GSM7034513 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314690 GSM7034514_r1 SRP421523 PRJNA932800 SRS16713446 GSM7034514 GSM7034514 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314691 GSM7034515_r1 SRP421523 PRJNA932800 SRS16713447 GSM7034515 GSM7034515 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314692 GSM7034516_r1 SRP421523 PRJNA932800 SRS16713448 GSM7034516 GSM7034516 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314693 GSM7034475_r1 SRP421523 PRJNA932800 SRS16713452 GSM7034475 GSM7034475 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314694 GSM7034476_r1 SRP421523 PRJNA932800 SRS16713451 GSM7034476 GSM7034476 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314695 GSM7034477_r1 SRP421523 PRJNA932800 SRS16713449 GSM7034477 GSM7034477 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314696 GSM7034478_r1 SRP421523 PRJNA932800 SRS16713450 GSM7034478 GSM7034478 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314697 GSM7034479_r1 SRP421523 PRJNA932800 SRS16713453 GSM7034479 GSM7034479 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314698 GSM7034480_r1 SRP421523 PRJNA932800 SRS16713454 GSM7034480 GSM7034480 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314699 GSM7034481_r1 SRP421523 PRJNA932800 SRS16713455 GSM7034481 GSM7034481 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314700 GSM7034482_r1 SRP421523 PRJNA932800 SRS16713456 GSM7034482 GSM7034482 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314701 GSM7034483_r1 SRP421523 PRJNA932800 SRS16713457 GSM7034483 GSM7034483 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314702 GSM7034484_r1 SRP421523 PRJNA932800 SRS16713458 GSM7034484 GSM7034484 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314703 GSM7034485_r1 SRP421523 PRJNA932800 SRS16713459 GSM7034485 GSM7034485 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314704 GSM7034486_r1 SRP421523 PRJNA932800 SRS16713460 GSM7034486 GSM7034486 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314705 GSM7034487_r1 SRP421523 PRJNA932800 SRS16713462 GSM7034487 GSM7034487 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314706 GSM7034488_r1 SRP421523 PRJNA932800 SRS16713461 GSM7034488 GSM7034488 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314707 GSM7034489_r1 SRP421523 PRJNA932800 SRS16713463 GSM7034489 GSM7034489 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000 SRX19314708 GSM7034490_r1 SRP421523 PRJNA932800 SRS16713464 GSM7034490 GSM7034490 RNA-Seq TRANSCRIPTOMIC cDNA Samples were lysed in TRIzol and total RNA was extracted using the DirectZOL Microprep kit (Zymo Research). For SLAM conversion, RNA was mixed with 20 mM Iodacetamide (Thermo Fisher Scientific) and incubated at 50°C shaking for 30 min in the dark. The reaction was stopped by mixing with 100 µM dithiothreitol, and converted RNA was purified using the RNA Clean&Concentrator-5 (Zymo Research). 200 ng of converted RNA was used for constructing the libraries. RNA libraries for SLAM-seq were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) with oligo-dT beads for capture of poly-A-mRNA following manufacturer's protocols. Illumina NovaSeq 6000