SRX20256042 20230413_LS-ctrl-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 9 days post start of selection. Replicate 1 SRS17587653 20230413_LS-ctrl-D10-R1 20230413_LS-ctrl-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256043 20230413_LS-ctrl-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 9 days post start of selection. Replicate 2 SRS17587654 20230413_LS-ctrl-D10-R2 20230413_LS-ctrl-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256044 20230413_LS-ctrl-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 6 days post start of selection. Replicate 2 SRS17587655 20230413_LS-ctrl-D7-R2 20230413_LS-ctrl-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256045 20230413_LS-ctrl-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 6 days post start of selection. Replicate 3 SRS17587656 20230413_LS-ctrl-D7-R3 20230413_LS-ctrl-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256046 20230413_LS-p624-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 9 days post start of selection. Replicate 1 SRS17587657 20230413_LS-p624-D10-R1 20230413_LS-p624-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256047 20230413_LS-p624-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 9 days post start of selection. Replicate 2 SRS17587658 20230413_LS-p624-D10-R2 20230413_LS-p624-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256048 20230413_LS-p624-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 9 days post start of selection. Replicate 3 SRS17587659 20230413_LS-p624-D10-R3 20230413_LS-p624-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256049 20230413_LS-p624-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 2 days post start of selection. Replicate 1 SRS17587660 20230413_LS-p624-D3-R1 20230413_LS-p624-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256050 20230413_LS-p624-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 2 days post start of selection. Replicate 2 SRS17587661 20230413_LS-p624-D3-R2 20230413_LS-p624-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256051 20230413_LS-p624-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 2 days post start of selection. Replicate 3 SRS17587662 20230413_LS-p624-D3-R3 20230413_LS-p624-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256052 20230413_LS-p624-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 4 days post start of selection. Replicate 1 SRS17587663 20230413_LS-p624-D5-R1 20230413_LS-p624-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256053 20230413_LS-p624-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 4 days post start of selection. Replicate 2 SRS17587664 20230413_LS-p624-D5-R2 20230413_LS-p624-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256054 20230413_LS-ctrl-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 9 days post start of selection. Replicate 3 SRS17587665 20230413_LS-ctrl-D10-R3 20230413_LS-ctrl-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256055 20230413_LS-p624-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 4 days post start of selection. Replicate 3 SRS17587666 20230413_LS-p624-D5-R3 20230413_LS-p624-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256056 20230413_LS-p624-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 6 days post start of selection. Replicate 1 SRS17587667 20230413_LS-p624-D7-R1 20230413_LS-p624-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256057 20230413_LS-p624-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 6 days post start of selection. Replicate 2 SRS17587668 20230413_LS-p624-D7-R2 20230413_LS-p624-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256058 20230413_LS-p624-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Cas9. Harvested 6 days post start of selection. Replicate 3 SRS17587669 20230413_LS-p624-D7-R3 20230413_LS-p624-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256059 20230413_LS-p625-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 9 days post start of selection. Replicate 1 SRS17587670 20230413_LS-p625-D10-R1 20230413_LS-p625-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256060 20230413_LS-p625-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 9 days post start of selection. Replicate 2 SRS17587671 20230413_LS-p625-D10-R2 20230413_LS-p625-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256061 20230413_LS-p625-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 9 days post start of selection. Replicate 3 SRS17587672 20230413_LS-p625-D10-R3 20230413_LS-p625-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256062 20230413_LS-p625-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 2 days post start of selection. Replicate 1 SRS17587673 20230413_LS-p625-D3-R1 20230413_LS-p625-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256063 20230413_LS-p625-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 2 days post start of selection. Replicate 2 SRS17587674 20230413_LS-p625-D3-R2 20230413_LS-p625-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256064 20230413_LS-p625-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 2 days post start of selection. Replicate 3 SRS17587675 20230413_LS-p625-D3-R3 20230413_LS-p625-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256065 20230413_LS-ctrl-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 2 days post start of selection. Replicate 1 SRS17587676 20230413_LS-ctrl-D3-R1 20230413_LS-ctrl-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256066 20230413_LS-p625-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 4 days post start of selection. Replicate 1 SRS17587677 20230413_LS-p625-D5-R1 20230413_LS-p625-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256067 20230413_LS-p625-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 4 days post start of selection. Replicate 2 SRS17587678 20230413_LS-p625-D5-R2 20230413_LS-p625-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256068 20230413_LS-p625-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 4 days post start of selection. Replicate 3 SRS17587679 20230413_LS-p625-D5-R3 20230413_LS-p625-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256069 20230413_LS-p625-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 6 days post start of selection. Replicate 1 SRS17587680 20230413_LS-p625-D7-R1 20230413_LS-p625-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256070 20230413_LS-p625-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 6 days post start of selection. Replicate 2 SRS17587681 20230413_LS-p625-D7-R2 20230413_LS-p625-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256071 20230413_LS-p625-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-C.NR. Harvested 6 days post start of selection. Replicate 3 SRS17587682 20230413_LS-p625-D7-R3 20230413_LS-p625-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256072 20230413_LS-p632-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 9 days post start of selection. Replicate 1 SRS17587683 20230413_LS-p632-D10-R1 20230413_LS-p632-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256073 20230413_LS-p632-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 9 days post start of selection. Replicate 2 SRS17587684 20230413_LS-p632-D10-R2 20230413_LS-p632-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256074 20230413_LS-p632-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 9 days post start of selection. Replicate 3 SRS17587685 20230413_LS-p632-D10-R3 20230413_LS-p632-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256075 20230413_LS-p632-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 2 days post start of selection. Replicate 1 SRS17587686 20230413_LS-p632-D3-R1 20230413_LS-p632-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256076 20230413_LS-ctrl-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 2 days post start of selection. Replicate 2 SRS17587687 20230413_LS-ctrl-D3-R2 20230413_LS-ctrl-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256077 20230413_LS-p632-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 2 days post start of selection. Replicate 2 SRS17587688 20230413_LS-p632-D3-R2 20230413_LS-p632-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256078 20230413_LS-p632-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 2 days post start of selection. Replicate 3 SRS17587689 20230413_LS-p632-D3-R3 20230413_LS-p632-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256079 20230413_LS-p632-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 4 days post start of selection. Replicate 1 SRS17587690 20230413_LS-p632-D5-R1 20230413_LS-p632-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256080 20230413_LS-p632-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 4 days post start of selection. Replicate 2 SRS17587691 20230413_LS-p632-D5-R2 20230413_LS-p632-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256081 20230413_LS-p632-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 4 days post start of selection. Replicate 3 SRS17587692 20230413_LS-p632-D5-R3 20230413_LS-p632-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256082 20230413_LS-p632-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 6 days post start of selection. Replicate 1 SRS17587693 20230413_LS-p632-D7-R1 20230413_LS-p632-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256083 20230413_LS-p632-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 6 days post start of selection. Replicate 2 SRS17587694 20230413_LS-p632-D7-R2 20230413_LS-p632-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256084 20230413_LS-p632-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB. Harvested 6 days post start of selection. Replicate 3 SRS17587695 20230413_LS-p632-D7-R3 20230413_LS-p632-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256085 20230413_LS-p632-ctrl-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 9 days post start of selection. Replicate 1 SRS17587696 20230413_LS-p632-ctrl-D10-R1 20230413_LS-p632-ctrl-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256086 20230413_LS-p632-ctrl-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 9 days post start of selection. Replicate 2 SRS17587697 20230413_LS-p632-ctrl-D10-R2 20230413_LS-p632-ctrl-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256087 20230413_LS-ctrl-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 2 days post start of selection. Replicate 3 SRS17587698 20230413_LS-ctrl-D3-R3 20230413_LS-ctrl-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256088 20230413_LS-p632-ctrl-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 9 days post start of selection. Replicate 3 SRS17587699 20230413_LS-p632-ctrl-D10-R3 20230413_LS-p632-ctrl-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256089 20230413_LS-p632-ctrl-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 2 days post start of selection. Replicate 1 SRS17587700 20230413_LS-p632-ctrl-D3-R1 20230413_LS-p632-ctrl-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256090 20230413_LS-p632-ctrl-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 2 days post start of selection. Replicate 2 SRS17587701 20230413_LS-p632-ctrl-D3-R2 20230413_LS-p632-ctrl-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256091 20230413_LS-p632-ctrl-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 2 days post start of selection. Replicate 3 SRS17587702 20230413_LS-p632-ctrl-D3-R3 20230413_LS-p632-ctrl-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256092 20230413_LS-p632-ctrl-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 4 days post start of selection. Replicate 1 SRS17587703 20230413_LS-p632-ctrl-D5-R1 20230413_LS-p632-ctrl-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256093 20230413_LS-p632-ctrl-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 4 days post start of selection. Replicate 2 SRS17587704 20230413_LS-p632-ctrl-D5-R2 20230413_LS-p632-ctrl-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256094 20230413_LS-p632-ctrl-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 4 days post start of selection. Replicate 3 SRS17587705 20230413_LS-p632-ctrl-D5-R3 20230413_LS-p632-ctrl-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256095 20230413_LS-p632-ctrl-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 6 days post start of selection. Replicate 1 SRS17587706 20230413_LS-p632-ctrl-D7-R1 20230413_LS-p632-ctrl-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256096 20230413_LS-p632-ctrl-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 6 days post start of selection. Replicate 2 SRS17587707 20230413_LS-p632-ctrl-D7-R2 20230413_LS-p632-ctrl-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256097 20230413_LS-p632-ctrl-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with TnpB-Control Plasmid. Harvested 6 days post start of selection. Replicate 3 SRS17587708 20230413_LS-p632-ctrl-D7-R3 20230413_LS-p632-ctrl-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256098 20230413_LS-ctrl-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 4 days post start of selection. Replicate 1 SRS17587709 20230413_LS-ctrl-D5-R1 20230413_LS-ctrl-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256099 20230413_LS-p633-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 9 days post start of selection. Replicate 1 SRS17587710 20230413_LS-p633-D10-R1 20230413_LS-p633-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256100 20230413_LS-p633-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 9 days post start of selection. Replicate 2 SRS17587711 20230413_LS-p633-D10-R2 20230413_LS-p633-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256101 20230413_LS-p633-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 9 days post start of selection. Replicate 3 SRS17587712 20230413_LS-p633-D10-R3 20230413_LS-p633-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256102 20230413_LS-p633-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 2 days post start of selection. Replicate 1 SRS17587713 20230413_LS-p633-D3-R1 20230413_LS-p633-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256103 20230413_LS-p633-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 2 days post start of selection. Replicate 2 SRS17587714 20230413_LS-p633-D3-R2 20230413_LS-p633-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256104 20230413_LS-p633-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 2 days post start of selection. Replicate 3 SRS17587715 20230413_LS-p633-D3-R3 20230413_LS-p633-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256105 20230413_LS-p633-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 4 days post start of selection. Replicate 1 SRS17587716 20230413_LS-p633-D5-R1 20230413_LS-p633-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256106 20230413_LS-p633-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 4 days post start of selection. Replicate 2 SRS17587717 20230413_LS-p633-D5-R2 20230413_LS-p633-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256107 20230413_LS-p633-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 4 days post start of selection. Replicate 3 SRS17587718 20230413_LS-p633-D5-R3 20230413_LS-p633-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256108 20230413_LS-p633-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 6 days post start of selection. Replicate 1 SRS17587719 20230413_LS-p633-D7-R1 20230413_LS-p633-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256109 20230413_LS-ctrl-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 4 days post start of selection. Replicate 2 SRS17587720 20230413_LS-ctrl-D5-R2 20230413_LS-ctrl-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256110 20230413_LS-p633-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 6 days post start of selection. Replicate 2 SRS17587721 20230413_LS-p633-D7-R2 20230413_LS-p633-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256111 20230413_LS-p633-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI. Harvested 6 days post start of selection. Replicate 3 SRS17587722 20230413_LS-p633-D7-R3 20230413_LS-p633-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256112 20230413_LS-p633-ctrl-D10-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 9 days post start of selection. Replicate 1 SRS17587723 20230413_LS-p633-ctrl-D10-R1 20230413_LS-p633-ctrl-D10-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256113 20230413_LS-p633-ctrl-D10-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 9 days post start of selection. Replicate 2 SRS17587724 20230413_LS-p633-ctrl-D10-R2 20230413_LS-p633-ctrl-D10-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256114 20230413_LS-p633-ctrl-D10-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 9 days post start of selection. Replicate 3 SRS17587725 20230413_LS-p633-ctrl-D10-R3 20230413_LS-p633-ctrl-D10-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256115 20230413_LS-p633-ctrl-D3-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 2 days post start of selection. Replicate 1 SRS17587726 20230413_LS-p633-ctrl-D3-R1 20230413_LS-p633-ctrl-D3-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256116 20230413_LS-p633-ctrl-D3-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 2 days post start of selection. Replicate 2 SRS17587727 20230413_LS-p633-ctrl-D3-R2 20230413_LS-p633-ctrl-D3-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256117 20230413_LS-p633-ctrl-D3-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 2 days post start of selection. Replicate 3 SRS17587728 20230413_LS-p633-ctrl-D3-R3 20230413_LS-p633-ctrl-D3-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256118 20230413_LS-p633-ctrl-D5-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 4 days post start of selection. Replicate 1 SRS17587729 20230413_LS-p633-ctrl-D5-R1 20230413_LS-p633-ctrl-D5-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256119 20230413_LS-p633-ctrl-D5-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 4 days post start of selection. Replicate 2 SRS17587730 20230413_LS-p633-ctrl-D5-R2 20230413_LS-p633-ctrl-D5-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256120 20230413_LS-ctrl-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 4 days post start of selection. Replicate 3 SRS17587731 20230413_LS-ctrl-D5-R3 20230413_LS-ctrl-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256121 20230413_LS-p633-ctrl-D5-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 4 days post start of selection. Replicate 3 SRS17587732 20230413_LS-p633-ctrl-D5-R3 20230413_LS-p633-ctrl-D5-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256122 20230413_LS-p633-ctrl-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 6 days post start of selection. Replicate 1 SRS17587733 20230413_LS-p633-ctrl-D7-R1 20230413_LS-p633-ctrl-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256123 20230413_LS-p633-ctrl-D7-R2 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 6 days post start of selection. Replicate 2 SRS17587734 20230413_LS-p633-ctrl-D7-R2 20230413_LS-p633-ctrl-D7-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256124 20230413_LS-p633-ctrl-D7-R3 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with CasMINI-Control Plasmid. Harvested 6 days post start of selection. Replicate 3 SRS17587735 20230413_LS-p633-ctrl-D7-R3 20230413_LS-p633-ctrl-D7-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256125 LS-p595ctrl-R1 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with CjCas9-TadCBd. Replicate: 1 SRS17587736 LS-p595ctrl-R1 LS-p595ctrl-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256126 LS-p595ctrl-R2 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with CjCas9-TadCBd. Replicate: 2 SRS17587737 LS-p595ctrl-R2 LS-p595ctrl-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256127 LS-p595ctrl-R3 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with CjCas9-TadCBd. Replicate: 3 SRS17587738 LS-p595ctrl-R3 LS-p595ctrl-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256128 LS-p595evo-R1 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with evoCjCas9-TadCBd. Replicate: 1 SRS17587739 LS-p595evo-R1 LS-p595evo-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256129 LS-p595evo-R2 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with evoCjCas9-TadCBd. Replicate: 2 SRS17587740 LS-p595evo-R2 LS-p595evo-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256130 LS-p595evo-R3 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with evoCjCas9-TadCBd. Replicate: 3 SRS17587741 LS-p595evo-R3 LS-p595evo-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256131 20230413_LS-ctrl-D7-R1 SRP404862 PRJNA893560 PCR amplification of Nuclease library treated with Nme2-Control Plasmid. Harvested 6 days post start of selection. Replicate 1 SRS17587742 20230413_LS-ctrl-D7-R1 20230413_LS-ctrl-D7-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256132 LS-p595wlt-R1 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with control Plasmid. Replicate: 1 SRS17587743 LS-p595wlt-R1 LS-p595wlt-R1 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256133 LS-p595wlt-R2 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with control Plasmid. Replicate: 2 SRS17587744 LS-p595wlt-R2 LS-p595wlt-R2 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX20256134 LS-p595wlt-R3 SRP404862 PRJNA893560 PCR amplification of nuclease library treated with control Plasmid. Replicate: 3 SRS17587745 LS-p595wlt-R3 LS-p595wlt-R3 AMPLICON SYNTHETIC PCR Illumina MiSeq