SRX20256036 GSM7306431_r1 SRP436502 PRJNA970295 SRS17587647 GSM7306431 GSM7306431 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Thyroid tissues dissected from postnatal days 5, 10, 20, and 30 C57 mice were washed twice in 1 × PBS (Gibco) and cut into 1 mm3 pieces. Tissue pieces were then enzymatically digested with 3 mL digestion medium containing 5 mg/mL collagenase I/II, 0.2 u/mL dispase (Gibco), and 0.1 mg/mL DNAase. These samples were subsequently incubated at 37°C for 30 min in a water bath shaker, and the suspended cells were washed with 20% fetal bovine serum (FBS) in Dulbecco's modified Eagle's medium (DMEM), filtered through a 40 µm Cell-Strainer nylon mesh (BD), and centrifuged at 700 × g for 10 min. After washing with MACS buffer, dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec, Bielefeld, Germany), and the samples with cell viability > 90%, as assessed by trypan blue staining, were passed for initial quality control. Sample libraries were prepared according to the Chromium Single Cell 3' Library and Gel Bead Kit instructions (v2-v3) and were sequenced on an Illumina NovaSeq 6000 System. The gene-barcode matrices were generated using CellRanger's (v2-4) recommended pipeline, which aligned the droplet-based sequencing data against the mouse mm10 reference genome and counted the UMIs for each cell. The output was a count matrix containing all UMI counts for each droplet. Sequence alignment and transcript counts were performed using the CellRanger software. Illumina NovaSeq 6000 SRX20256037 GSM7306432_r1 SRP436502 PRJNA970295 SRS17587648 GSM7306432 GSM7306432 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Thyroid tissues dissected from postnatal days 5, 10, 20, and 30 C57 mice were washed twice in 1 × PBS (Gibco) and cut into 1 mm3 pieces. Tissue pieces were then enzymatically digested with 3 mL digestion medium containing 5 mg/mL collagenase I/II, 0.2 u/mL dispase (Gibco), and 0.1 mg/mL DNAase. These samples were subsequently incubated at 37°C for 30 min in a water bath shaker, and the suspended cells were washed with 20% fetal bovine serum (FBS) in Dulbecco's modified Eagle's medium (DMEM), filtered through a 40 µm Cell-Strainer nylon mesh (BD), and centrifuged at 700 × g for 10 min. After washing with MACS buffer, dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec, Bielefeld, Germany), and the samples with cell viability > 90%, as assessed by trypan blue staining, were passed for initial quality control. Sample libraries were prepared according to the Chromium Single Cell 3' Library and Gel Bead Kit instructions (v2-v3) and were sequenced on an Illumina NovaSeq 6000 System. The gene-barcode matrices were generated using CellRanger's (v2-4) recommended pipeline, which aligned the droplet-based sequencing data against the mouse mm10 reference genome and counted the UMIs for each cell. The output was a count matrix containing all UMI counts for each droplet. Sequence alignment and transcript counts were performed using the CellRanger software. Illumina NovaSeq 6000 SRX20256038 GSM7306433_r1 SRP436502 PRJNA970295 SRS17587649 GSM7306433 GSM7306433 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Thyroid tissues dissected from postnatal days 5, 10, 20, and 30 C57 mice were washed twice in 1 × PBS (Gibco) and cut into 1 mm3 pieces. Tissue pieces were then enzymatically digested with 3 mL digestion medium containing 5 mg/mL collagenase I/II, 0.2 u/mL dispase (Gibco), and 0.1 mg/mL DNAase. These samples were subsequently incubated at 37°C for 30 min in a water bath shaker, and the suspended cells were washed with 20% fetal bovine serum (FBS) in Dulbecco's modified Eagle's medium (DMEM), filtered through a 40 µm Cell-Strainer nylon mesh (BD), and centrifuged at 700 × g for 10 min. After washing with MACS buffer, dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec, Bielefeld, Germany), and the samples with cell viability > 90%, as assessed by trypan blue staining, were passed for initial quality control. Sample libraries were prepared according to the Chromium Single Cell 3' Library and Gel Bead Kit instructions (v2-v3) and were sequenced on an Illumina NovaSeq 6000 System. The gene-barcode matrices were generated using CellRanger's (v2-4) recommended pipeline, which aligned the droplet-based sequencing data against the mouse mm10 reference genome and counted the UMIs for each cell. The output was a count matrix containing all UMI counts for each droplet. Sequence alignment and transcript counts were performed using the CellRanger software. Illumina NovaSeq 6000 SRX20256039 GSM7306434_r1 SRP436502 PRJNA970295 SRS17587650 GSM7306434 GSM7306434 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Thyroid tissues dissected from postnatal days 5, 10, 20, and 30 C57 mice were washed twice in 1 × PBS (Gibco) and cut into 1 mm3 pieces. Tissue pieces were then enzymatically digested with 3 mL digestion medium containing 5 mg/mL collagenase I/II, 0.2 u/mL dispase (Gibco), and 0.1 mg/mL DNAase. These samples were subsequently incubated at 37°C for 30 min in a water bath shaker, and the suspended cells were washed with 20% fetal bovine serum (FBS) in Dulbecco's modified Eagle's medium (DMEM), filtered through a 40 µm Cell-Strainer nylon mesh (BD), and centrifuged at 700 × g for 10 min. After washing with MACS buffer, dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec, Bielefeld, Germany), and the samples with cell viability > 90%, as assessed by trypan blue staining, were passed for initial quality control. Sample libraries were prepared according to the Chromium Single Cell 3' Library and Gel Bead Kit instructions (v2-v3) and were sequenced on an Illumina NovaSeq 6000 System. The gene-barcode matrices were generated using CellRanger's (v2-4) recommended pipeline, which aligned the droplet-based sequencing data against the mouse mm10 reference genome and counted the UMIs for each cell. The output was a count matrix containing all UMI counts for each droplet. Sequence alignment and transcript counts were performed using the CellRanger software. Illumina NovaSeq 6000