SRX20313453
GSM7333219_r1
GSM7333219: sham, control, replicate 1; Mus musculus; RNA-Seq
SRP437453
PRJNA971964
SRS17636433
GSM7333219
GSM7333219
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
Four days after stroke onset, brains were removed and sliced in ice-cold artificial cerebrospinal fluid. Peri-infarct area was trimmed and treated with pronase (Sigma-Aldrich) for 75 minutes at room temperature. Enzyme-treated tissue was dissociated by gentle pipetting with a Pasteur pipette. Neuron enriched cell populations (Thy1+/ACSA2-/CD31-/CD45-/O4-/CD140a-/CD140b-) were collected by fluorescence-activated cell sorting (BD FACS Aria III). RNA libraries for scRNA-seq were prepared using Chromium Next GEM Single-cell 3' Reagent Kits v3.1 (10x Genomics) according to the manufacturer's protocol.
DNBSEQ-G400
SRX20313454
GSM7333220_r1
GSM7333220: day 4, control, replicate 1; Mus musculus; RNA-Seq
SRP437453
PRJNA971964
SRS17636434
GSM7333220
GSM7333220
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
Four days after stroke onset, brains were removed and sliced in ice-cold artificial cerebrospinal fluid. Peri-infarct area was trimmed and treated with pronase (Sigma-Aldrich) for 75 minutes at room temperature. Enzyme-treated tissue was dissociated by gentle pipetting with a Pasteur pipette. Neuron enriched cell populations (Thy1+/ACSA2-/CD31-/CD45-/O4-/CD140a-/CD140b-) were collected by fluorescence-activated cell sorting (BD FACS Aria III). RNA libraries for scRNA-seq were prepared using Chromium Next GEM Single-cell 3' Reagent Kits v3.1 (10x Genomics) according to the manufacturer's protocol.
DNBSEQ-G400
SRX20313455
GSM7333221_r1
GSM7333221: day 4, control, replicate 2; Mus musculus; RNA-Seq
SRP437453
PRJNA971964
SRS17636435
GSM7333221
GSM7333221
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
Four days after stroke onset, brains were removed and sliced in ice-cold artificial cerebrospinal fluid. Peri-infarct area was trimmed and treated with pronase (Sigma-Aldrich) for 75 minutes at room temperature. Enzyme-treated tissue was dissociated by gentle pipetting with a Pasteur pipette. Neuron enriched cell populations (Thy1+/ACSA2-/CD31-/CD45-/O4-/CD140a-/CD140b-) were collected by fluorescence-activated cell sorting (BD FACS Aria III). RNA libraries for scRNA-seq were prepared using Chromium Next GEM Single-cell 3' Reagent Kits v3.1 (10x Genomics) according to the manufacturer's protocol.
DNBSEQ-G400
SRX20313456
GSM7333222_r1
GSM7333222: day 4, cKO, replicate 1; Mus musculus; RNA-Seq
SRP437453
PRJNA971964
SRS17636436
GSM7333222
GSM7333222
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
Four days after stroke onset, brains were removed and sliced in ice-cold artificial cerebrospinal fluid. Peri-infarct area was trimmed and treated with pronase (Sigma-Aldrich) for 75 minutes at room temperature. Enzyme-treated tissue was dissociated by gentle pipetting with a Pasteur pipette. Neuron enriched cell populations (Thy1+/ACSA2-/CD31-/CD45-/O4-/CD140a-/CD140b-) were collected by fluorescence-activated cell sorting (BD FACS Aria III). RNA libraries for scRNA-seq were prepared using Chromium Next GEM Single-cell 3' Reagent Kits v3.1 (10x Genomics) according to the manufacturer's protocol.
DNBSEQ-G400
SRX20313457
GSM7333223_r1
GSM7333223: day 4, cKO, replicate 2; Mus musculus; RNA-Seq
SRP437453
PRJNA971964
SRS17636437
GSM7333223
GSM7333223
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
Four days after stroke onset, brains were removed and sliced in ice-cold artificial cerebrospinal fluid. Peri-infarct area was trimmed and treated with pronase (Sigma-Aldrich) for 75 minutes at room temperature. Enzyme-treated tissue was dissociated by gentle pipetting with a Pasteur pipette. Neuron enriched cell populations (Thy1+/ACSA2-/CD31-/CD45-/O4-/CD140a-/CD140b-) were collected by fluorescence-activated cell sorting (BD FACS Aria III). RNA libraries for scRNA-seq were prepared using Chromium Next GEM Single-cell 3' Reagent Kits v3.1 (10x Genomics) according to the manufacturer's protocol.
DNBSEQ-G400