SRX22292081 GSM7872697_r1 SRP469282 PRJNA1033783 SRS19343089 GSM7872697 GSM7872697 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Female adult mosquitoes at 7-10 days after eclosion were anesthetized on ice. Subsequently, midguts were dissected from a group of 40 mosquitoes, then washed and temporarily stored in a microtube filled with Schneider's Drosophila Medium kept on ice. After the dissection, the medium was discarded, and 400 μl of dissociation buffer was added into the microtube. The mixture was incubated on a rotator for 20-30 min at room temperature. Then, 400 μl of Schneider's Drosophila Medium was added to the microtube and mixed well. A total of 800 μl of the cell suspension was filtered with a 40 μm Flowmi® Cell Strainer to remove larger cell debris. To remove impurities and smaller cell debris, the filter was centrifuged over a density of approx. of 1.12 g/ml made with OptiPrep™ at 400× g RCF, 4 ℃ for 20 min. Viable cell band was collected using a 200 μl pipette tip, mixed well in a new microtube and centrifuged at 400× g for 20 min again. The cell precipitation was washed with 1× PBS for several times and then resuspended with 200 μl of Schneider's Drosophila Medium. Library construction was performed with Single Cell 3' v3 Chemistry according to the manufacturer's instructions. Illumina NovaSeq 6000 SRX22292080 GSM7872696_r1 SRP469282 PRJNA1033783 SRS19343088 GSM7872696 GSM7872696 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Female adult mosquitoes at 7-10 days after eclosion were anesthetized on ice. Subsequently, midguts were dissected from a group of 40 mosquitoes, then washed and temporarily stored in a microtube filled with Schneider's Drosophila Medium kept on ice. After the dissection, the medium was discarded, and 400 μl of dissociation buffer was added into the microtube. The mixture was incubated on a rotator for 20-30 min at room temperature. Then, 400 μl of Schneider's Drosophila Medium was added to the microtube and mixed well. A total of 800 μl of the cell suspension was filtered with a 40 μm Flowmi® Cell Strainer to remove larger cell debris. To remove impurities and smaller cell debris, the filter was centrifuged over a density of approx. of 1.12 g/ml made with OptiPrep™ at 400× g RCF, 4 ℃ for 20 min. Viable cell band was collected using a 200 μl pipette tip, mixed well in a new microtube and centrifuged at 400× g for 20 min again. The cell precipitation was washed with 1× PBS for several times and then resuspended with 200 μl of Schneider's Drosophila Medium. Library construction was performed with Single Cell 3' v3 Chemistry according to the manufacturer's instructions. Illumina NovaSeq 6000