SRX22322000
GSM7874574_r1
GSM7874574: Schmidtea mediterranea, whole-body, scRNA-seq, batch 1, library 15_5_shallow, library 23_1_deep; Schmidtea mediterranea; RNA-Seq
SRP469511
PRJNA1034119
SRS19372700
GSM7874574
GSM7874574
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
We used ACME dissociation to dissociate whole animals into fixed and permeable cell suspensions. Dissociated cells were proccessed using a SPLiT-seq protocol (we sorted singlets by FACS during SPLiT-seq, right before the cell lysis step). We followed the SPLiT-seq library construction pipeline (Dynabeads cDNA purification, template switching, PCR amplification, size selection, tagmentation, indexed-PCR amplification and second size selection). Size selection was performed using Roche KAPA Pure Beads at 0.6X-0.8X ratios. Tagmentation was performed using the Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer´s instructions.
Illumina NovaSeq 6000
SRX22322001
GSM7874575_r1
GSM7874575: Schmidtea mediterranea, whole-body, scRNA-seq, batch 1, library 15_6_shallow, library 23_2_deep; Schmidtea mediterranea; RNA-Seq
SRP469511
PRJNA1034119
SRS19372701
GSM7874575
GSM7874575
RNA-Seq
TRANSCRIPTOMIC SINGLE CELL
cDNA
We used ACME dissociation to dissociate whole animals into fixed and permeable cell suspensions. Dissociated cells were proccessed using a SPLiT-seq protocol (we sorted singlets by FACS during SPLiT-seq, right before the cell lysis step). We followed the SPLiT-seq library construction pipeline (Dynabeads cDNA purification, template switching, PCR amplification, size selection, tagmentation, indexed-PCR amplification and second size selection). Size selection was performed using Roche KAPA Pure Beads at 0.6X-0.8X ratios. Tagmentation was performed using the Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer´s instructions.
Illumina NovaSeq 6000