SRX22339433 GSM7879998_r1 SRP469849 PRJNA1034986 SRS19389082 GSM7879998 GSM7879998 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Neutrophils were enriched from the lung of Balb/c animals infected with NTHi using magnetic beads. Single cells from the enriched neutrophil fraction were stained with pooled BioLegend TotalSeq-B antibodies. Stained cells were diluted in PBS with a final concentration of 1000 cells per µL. Stained cells were loaded into a single lane of 10X Chromium (Single Cell 3' V3). Library construction for both polyA RNA and surface protin was done using 10x Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 with Feature Barcoding technology protocol Illumina NovaSeq 6000 SRX22339434 GSM7879999_r1 SRP469849 PRJNA1034986 SRS19389083 GSM7879999 GSM7879999 RNA-Seq OTHER cDNA Neutrophils were enriched from the lung of Balb/c animals infected with NTHi using magnetic beads. Single cells from the enriched neutrophil fraction were stained with pooled BioLegend TotalSeq-B antibodies. Stained cells were diluted in PBS with a final concentration of 1000 cells per µL. Stained cells were loaded into a single lane of 10X Chromium (Single Cell 3' V3). Library construction for both polyA RNA and surface protin was done using 10x Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 with Feature Barcoding technology protocol Illumina NovaSeq 6000