SRX22366868 GSM7881931_r1 SRP470124 PRJNA1035517 SRS19414761 GSM7881931 GSM7881931 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA According to the manufacturer's instructions for the Chromium Next GEM Single Cell 3' Reagent Kit v3.1(10x Genomics), single cell sequencing libraries were constructed. The cell suspension was loaded onto the Chromium Next GEM Chip G and ran the Chromium Controller to generate single-cell gel beads in the emulsions (GEMs) according to the manufacturer's recommendation. Captured cells were lysed and the released RNA was barcoded through reverse transcription in individual GEMs. Barcoded, full-length cDNA was generated and libraries were constructed according to the performer's protocol. Next, the Qubit 4.0 was used to detect the concentration of cDNA products and libraries; the Agilent 2100 was used to check the integrity of cDNA libraries; and q-PCR was used to accurately quantify the effective concentration of libraries. After passing library inspection, sequencing was performed on the Illumina NovaSeq 6000 PE15 with a sequencing depth of at least 50,000 reads per cell (performed by Biomarker Technologies Corporation, Beijing, China). Illumina NovaSeq 6000 SRX22366869 GSM7881932_r1 SRP470124 PRJNA1035517 SRS19414762 GSM7881932 GSM7881932 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA According to the manufacturer's instructions for the Chromium Next GEM Single Cell 3' Reagent Kit v3.1(10x Genomics), single cell sequencing libraries were constructed. The cell suspension was loaded onto the Chromium Next GEM Chip G and ran the Chromium Controller to generate single-cell gel beads in the emulsions (GEMs) according to the manufacturer's recommendation. Captured cells were lysed and the released RNA was barcoded through reverse transcription in individual GEMs. Barcoded, full-length cDNA was generated and libraries were constructed according to the performer's protocol. Next, the Qubit 4.0 was used to detect the concentration of cDNA products and libraries; the Agilent 2100 was used to check the integrity of cDNA libraries; and q-PCR was used to accurately quantify the effective concentration of libraries. After passing library inspection, sequencing was performed on the Illumina NovaSeq 6000 PE15 with a sequencing depth of at least 50,000 reads per cell (performed by Biomarker Technologies Corporation, Beijing, China). Illumina NovaSeq 6000