SRX22382737 GSM7883138_r1 SRP470371 PRJNA1036208 SRS19428972 GSM7883138 GSM7883138 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382738 GSM7883139_r1 SRP470371 PRJNA1036208 SRS19428973 GSM7883139 GSM7883139 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382739 GSM7883140_r1 SRP470371 PRJNA1036208 SRS19428974 GSM7883140 GSM7883140 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382740 GSM7883141_r1 SRP470371 PRJNA1036208 SRS19428975 GSM7883141 GSM7883141 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382741 GSM7883142_r1 SRP470371 PRJNA1036208 SRS19428976 GSM7883142 GSM7883142 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382742 GSM7883143_r1 SRP470371 PRJNA1036208 SRS19428977 GSM7883143 GSM7883143 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382743 GSM7883144_r1 SRP470371 PRJNA1036208 SRS19428978 GSM7883144 GSM7883144 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382744 GSM7883145_r1 SRP470371 PRJNA1036208 SRS19428979 GSM7883145 GSM7883145 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382745 GSM7883146_r1 SRP470371 PRJNA1036208 SRS19428980 GSM7883146 GSM7883146 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382746 GSM7883147_r1 SRP470371 PRJNA1036208 SRS19428981 GSM7883147 GSM7883147 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382747 GSM7883148_r1 SRP470371 PRJNA1036208 SRS19428982 GSM7883148 GSM7883148 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382748 GSM7883149_r1 SRP470371 PRJNA1036208 SRS19428983 GSM7883149 GSM7883149 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382749 GSM7883150_r1 SRP470371 PRJNA1036208 SRS19428984 GSM7883150 GSM7883150 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382750 GSM7883151_r1 SRP470371 PRJNA1036208 SRS19428985 GSM7883151 GSM7883151 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382751 GSM7883152_r1 SRP470371 PRJNA1036208 SRS19428986 GSM7883152 GSM7883152 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382752 GSM7883153_r1 SRP470371 PRJNA1036208 SRS19428987 GSM7883153 GSM7883153 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382753 GSM7883154_r1 SRP470371 PRJNA1036208 SRS19428988 GSM7883154 GSM7883154 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382754 GSM7883155_r1 SRP470371 PRJNA1036208 SRS19428989 GSM7883155 GSM7883155 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382755 GSM7883156_r1 SRP470371 PRJNA1036208 SRS19428990 GSM7883156 GSM7883156 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382756 GSM7883157_r1 SRP470371 PRJNA1036208 SRS19428991 GSM7883157 GSM7883157 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382757 GSM7883158_r1 SRP470371 PRJNA1036208 SRS19428992 GSM7883158 GSM7883158 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382758 GSM7883159_r1 SRP470371 PRJNA1036208 SRS19428993 GSM7883159 GSM7883159 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382759 GSM7883160_r1 SRP470371 PRJNA1036208 SRS19428994 GSM7883160 GSM7883160 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550 SRX22382760 GSM7883161_r1 SRP470371 PRJNA1036208 SRS19428995 GSM7883161 GSM7883161 RNA-Seq TRANSCRIPTOMIC cDNA Cells/organoids were lysed in RLT buffer, then processed for RNA extraction, or flash frozen, and stored in -80oC until future extraction. RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research) The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina). The library construction used Nextera XT chemistry which tagmented 200pg of DNA and added adapters in one step. The fragmented and tagged DNA was then PCR amplified for 12 cycles to incorporate dual-indexing sequencing adapters to generate the final library. A combination of both concentration and library size patterning was used as QC metrics to determine the success of the assay. The UV Qubit fluorometer quantitation step determined the concentration of the library. The Bio/Fragment Analyzer trace revealed the size distribution and concentration of the final sequencing library. Based on these three parameters we assigned library scores to each library. Libraries with a pass score were then used for sequencing in NextSeq 550. NextSeq 550