SRX22386348 GSM7883276_r1 SRP470438 PRJNA1036218 SRS19432497 GSM7883276 GSM7883276 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA OVCA420 cells were washed with PBS then incubated with trypsin for 5 minutes and quenched using normal growth media (DMEM + 10% FBS) . The suspension was filtered, and large aggregates were further disrupted using a syringe plunger on a 70μm filter in order to generate a single-cell suspension. Cells were counted to assess viability (Trypan-blue exclusion) prior to staining. Single-cell suspensions were processed using the 10x Genomics Single Cell 3' RNA-seq kit (v3), loaded to target a yield of 10,000 cells per sample. Cell cDNA libraries were prepared according to the manufacturer's protocol and libraries were assessed using the Fragment Analyzer (Agilent). 3' scRNA-seq Illumina HiSeq 4000 SRX22386349 GSM7883277_r1 SRP470438 PRJNA1036218 SRS19432498 GSM7883277 GSM7883277 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA OVCA420 cells were washed with PBS then incubated with trypsin for 5 minutes and quenched using normal growth media (DMEM + 10% FBS) . The suspension was filtered, and large aggregates were further disrupted using a syringe plunger on a 70μm filter in order to generate a single-cell suspension. Cells were counted to assess viability (Trypan-blue exclusion) prior to staining. Single-cell suspensions were processed using the 10x Genomics Single Cell 3' RNA-seq kit (v3), loaded to target a yield of 10,000 cells per sample. Cell cDNA libraries were prepared according to the manufacturer's protocol and libraries were assessed using the Fragment Analyzer (Agilent). 3' scRNA-seq Illumina HiSeq 4000 SRX22386350 GSM7883278_r1 SRP470438 PRJNA1036218 SRS19432499 GSM7883278 GSM7883278 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA OVCA420 cells were washed with PBS then incubated with trypsin for 5 minutes and quenched using normal growth media (DMEM + 10% FBS) . The suspension was filtered, and large aggregates were further disrupted using a syringe plunger on a 70μm filter in order to generate a single-cell suspension. Cells were counted to assess viability (Trypan-blue exclusion) prior to staining. Single-cell suspensions were processed using the 10x Genomics Single Cell 3' RNA-seq kit (v3), loaded to target a yield of 10,000 cells per sample. Cell cDNA libraries were prepared according to the manufacturer's protocol and libraries were assessed using the Fragment Analyzer (Agilent). 3' scRNA-seq Illumina HiSeq 4000 SRX22386351 GSM7883279_r1 SRP470438 PRJNA1036218 SRS19432500 GSM7883279 GSM7883279 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA OVCA420 cells were washed with PBS then incubated with trypsin for 5 minutes and quenched using normal growth media (DMEM + 10% FBS) . The suspension was filtered, and large aggregates were further disrupted using a syringe plunger on a 70μm filter in order to generate a single-cell suspension. Cells were counted to assess viability (Trypan-blue exclusion) prior to staining. Single-cell suspensions were processed using the 10x Genomics Single Cell 3' RNA-seq kit (v3), loaded to target a yield of 10,000 cells per sample. Cell cDNA libraries were prepared according to the manufacturer's protocol and libraries were assessed using the Fragment Analyzer (Agilent). 3' scRNA-seq Illumina HiSeq 4000