SRX22388241 GSM7882764_r1 SRP470457 PRJNA1036177 SRS19432845 GSM7882764 GSM7882764 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The samples were fully lysed using a lysis buffer (10 mM Tris [pH 7.4], 10 mM NaCl, 3 mM MgCl2, and 0.05% (v/v) NP-40 detergent) and were then filtered by a 30 μm cell strainer. After pelleting, the nuclei were reconstituted in a wash buffer (10 mM Tris [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 1% BSA, 1 mM DTT, RNase inhibitor 1U/µl, nuclease-free water) and combined with 25% optiprep and layered on a 29% optiprep cushion. After centrifugation, the nuclei were reconstituted with the wash buffer again. Finally, the nuclei were resuspended into the nuclei resuspension buffer (nuclei buffer (10XGenomics, 20x) 1x, 1 mM DTT, RNase inhibitor 1U/µl, nuclease-free water) with a concentration of ~1x10^6/ml, and the single-cell nuclei suspension was prepared. Once the extracted nuclei passed the quality test, single-cell RNA sequencing (RNA-seq) libraries were prepared with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 on the Chromium Controller (10 × Genomics). Single-nuclei gel beads in the emulsion (GEMs) were produced as directed by the manufacturer. The released RNA of single nuclei was barcoded in each GEM via reverse transcription. Sequencing was performed with a depth of at least half a million reads/cell and 150 bp paired-end reads (PE150) using the Illumina NovaSeq 6000. Illumina NovaSeq 6000 SRX22388242 GSM7882765_r1 SRP470457 PRJNA1036177 SRS19432846 GSM7882765 GSM7882765 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The samples were fully lysed using a lysis buffer (10 mM Tris [pH 7.4], 10 mM NaCl, 3 mM MgCl2, and 0.05% (v/v) NP-40 detergent) and were then filtered by a 30 μm cell strainer. After pelleting, the nuclei were reconstituted in a wash buffer (10 mM Tris [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 1% BSA, 1 mM DTT, RNase inhibitor 1U/µl, nuclease-free water) and combined with 25% optiprep and layered on a 29% optiprep cushion. After centrifugation, the nuclei were reconstituted with the wash buffer again. Finally, the nuclei were resuspended into the nuclei resuspension buffer (nuclei buffer (10XGenomics, 20x) 1x, 1 mM DTT, RNase inhibitor 1U/µl, nuclease-free water) with a concentration of ~1x10^6/ml, and the single-cell nuclei suspension was prepared. Once the extracted nuclei passed the quality test, single-cell RNA sequencing (RNA-seq) libraries were prepared with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 on the Chromium Controller (10 × Genomics). Single-nuclei gel beads in the emulsion (GEMs) were produced as directed by the manufacturer. The released RNA of single nuclei was barcoded in each GEM via reverse transcription. Sequencing was performed with a depth of at least half a million reads/cell and 150 bp paired-end reads (PE150) using the Illumina NovaSeq 6000. Illumina NovaSeq 6000 SRX22388243 GSM7882766_r1 SRP470457 PRJNA1036177 SRS19432847 GSM7882766 GSM7882766 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The samples were fully lysed using a lysis buffer (10 mM Tris [pH 7.4], 10 mM NaCl, 3 mM MgCl2, and 0.05% (v/v) NP-40 detergent) and were then filtered by a 30 μm cell strainer. After pelleting, the nuclei were reconstituted in a wash buffer (10 mM Tris [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 1% BSA, 1 mM DTT, RNase inhibitor 1U/µl, nuclease-free water) and combined with 25% optiprep and layered on a 29% optiprep cushion. After centrifugation, the nuclei were reconstituted with the wash buffer again. Finally, the nuclei were resuspended into the nuclei resuspension buffer (nuclei buffer (10XGenomics, 20x) 1x, 1 mM DTT, RNase inhibitor 1U/µl, nuclease-free water) with a concentration of ~1x10^6/ml, and the single-cell nuclei suspension was prepared. Once the extracted nuclei passed the quality test, single-cell RNA sequencing (RNA-seq) libraries were prepared with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 on the Chromium Controller (10 × Genomics). Single-nuclei gel beads in the emulsion (GEMs) were produced as directed by the manufacturer. The released RNA of single nuclei was barcoded in each GEM via reverse transcription. Sequencing was performed with a depth of at least half a million reads/cell and 150 bp paired-end reads (PE150) using the Illumina NovaSeq 6000. Illumina NovaSeq 6000