SRP470710 PRJNA1037038 GSE247273 Sequencing of the effect of mechanical stimulation on mesenchymal stem cells Mechanical force is a crucial external stimulus that plays a significant role in regulating bone structure and remodeling. Excessive loading of the bone and joint can lead to increased catabolism, chondrocyte necrosis, apoptosis and damage to the collagen network of bone(3–5). Osteoarthritis (OA), a degenerative osteoarticular disease, is associated with abnormal mechanical force stimulation, which can occur in various joints such as knee, temporomandibular joint [TMJ], shoulder and hip(6). Conversely, the absence of mechanical loading, such as prolonged bed rest or exposure to a microgravity environment in space, can result in a rapid decrease in bone mass and strength. Understanding how mechanical stimuli regulate bone homeostasis is crucial for exploring therapeutic strategies for bone metabolic diseases.Mesechymal stem cells (MSCs) act as the external force sensoring and compression-bearing elements.What we want to explore is how mechanical stimulation affects the genome changes of mesenchymal stem cells. Overall design: In order to explore the influence of mechanical stimulation on mesenchymal stem cells, we designed two groups: the control group and the mechanical stimulation group. The mechanical stimulation group was stimulated by large mechanical force for 24 hours, and the expression differences were compared. GSE247273