SRP471445 PRJNA1039586 Yeast rho- mutants Whole genome sequencing of Saccharomyces cerevisiae strains (derivatives of W303 genetic background) carrying deletions in mtDNA (rho- genotype) using Illumina sequencing platform. We sequenced 17 independently obtained mutant strains each derived from the same parental strain W303-1B (ade2-101 his3-11 trp1-1 can1-100 leu2-3,112, GAL, psi+, URA3 MATalpha) carrying different deletions in their mitochondrial genome. Additionally, we sequenced the strain with wild-type mtDNA sequence (rho+ genotype) and two strains completely lacking mtDNA (rho0 genotype).Strains were cultivated on reach medium (YPD = 1% yeast extract, 2% bacto-peptone, 2% glucose).