SRS19824723 SAMN38704061 4932 Saccharomyces cerevisiae To identify the causal mutation that led to increased expression noise in the evolved clones, we performed bulk segregant analysis by crossing the evolved cells with their ancestral cells and analyzing their F1 haploid progeny. We measured protein expression noise levels of both reporter genes in 64 F1 segregants and established an ancestral-like pool and an evolved-like pool from each evolved clone (Evo 2, Evo 3 and Evo 4). For each bulk, 20 progenies exhibiting a consistent phenotype across the three replicates were pooled and subjected to whole genome sequencing analysis. bioproject 1023389 PRJNA1023389 strain W303 isolation_source Laboratory collection_date 2021-03-18 geo_loc_name Taiwan sample_type Mix of Genomic DNA BioSampleModel Microbe, viral or environmental