SRX22883429 TC2 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2500 SRS19855771 TempControl2 TC2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883430 TC3 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2501 SRS19855772 TempControl3 TC3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883431 TC4 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2510 SRS19855773 TempControl4 TC4 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883432 TL1 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2511 SRS19855774 TempLow1 TL1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883433 TH5 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2512 SRS19855775 TempHigh5 TH5 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883434 TC6 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2513 SRS19855776 TempControl6 TC6 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883435 TL8 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2514 SRS19855777 TempLow8 TL8 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883436 TH7 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2515 SRS19855778 TempHigh7 TH7 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883437 TC1 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2516 SRS19855779 TempControl1 TC1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883438 TL4 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2517 SRS19855780 TempLow4 TL4 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883439 TL2 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2518 SRS19855781 TempLow2 TL2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883440 TH8 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2519 SRS19855782 TempHigh8 TH8 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883441 TL5 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2502 SRS19855783 TempLow5 TL5 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883442 TC5 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2503 SRS19855784 TempControl5 TC5 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883443 TL7 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2504 SRS19855785 TempLow7 TL7 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883444 TH1 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2505 SRS19855786 TempHigh1 TH1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883445 TH3 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2506 SRS19855787 TempHigh3 TH3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883446 TC7 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2507 SRS19855788 TempControl7 TC7 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883447 TL3 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2508 SRS19855789 TempLow3 TL3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX22883448 TH4 SRP477878 bp0 Whole oysters were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2509 SRS19855790 TempHigh4 TH4 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500