SRX23112193 GSM8004756_r1 SRP482140 PRJNA1061564 SRS20067064 GSM8004756 GSM8004756 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112194 GSM8004757_r1 SRP482140 PRJNA1061564 SRS20067065 GSM8004757 GSM8004757 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112195 GSM8004758_r1 SRP482140 PRJNA1061564 SRS20067066 GSM8004758 GSM8004758 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112196 GSM8004759_r1 SRP482140 PRJNA1061564 SRS20067067 GSM8004759 GSM8004759 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112197 GSM8004760_r1 SRP482140 PRJNA1061564 SRS20067068 GSM8004760 GSM8004760 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112198 GSM8004761_r1 SRP482140 PRJNA1061564 SRS20067069 GSM8004761 GSM8004761 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112199 GSM8004762_r1 SRP482140 PRJNA1061564 SRS20067070 GSM8004762 GSM8004762 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112200 GSM8004763_r1 SRP482140 PRJNA1061564 SRS20067071 GSM8004763 GSM8004763 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112201 GSM8004764_r1 SRP482140 PRJNA1061564 SRS20067072 GSM8004764 GSM8004764 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112202 GSM8004765_r1 SRP482140 PRJNA1061564 SRS20067073 GSM8004765 GSM8004765 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112203 GSM8004766_r1 SRP482140 PRJNA1061564 SRS20067074 GSM8004766 GSM8004766 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112204 GSM8004767_r1 SRP482140 PRJNA1061564 SRS20067075 GSM8004767 GSM8004767 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112205 GSM8004768_r1 SRP482140 PRJNA1061564 SRS20067076 GSM8004768 GSM8004768 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112206 GSM8004769_r1 SRP482140 PRJNA1061564 SRS20067077 GSM8004769 GSM8004769 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112207 GSM8004770_r1 SRP482140 PRJNA1061564 SRS20067078 GSM8004770 GSM8004770 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112208 GSM8004771_r1 SRP482140 PRJNA1061564 SRS20067079 GSM8004771 GSM8004771 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112209 GSM8004772_r1 SRP482140 PRJNA1061564 SRS20067080 GSM8004772 GSM8004772 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112210 GSM8004773_r1 SRP482140 PRJNA1061564 SRS20067081 GSM8004773 GSM8004773 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112211 GSM8004774_r1 SRP482140 PRJNA1061564 SRS20067082 GSM8004774 GSM8004774 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112212 GSM8004775_r1 SRP482140 PRJNA1061564 SRS20067083 GSM8004775 GSM8004775 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112213 GSM8004776_r1 SRP482140 PRJNA1061564 SRS20067084 GSM8004776 GSM8004776 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000 SRX23112214 GSM8004777_r1 SRP482140 PRJNA1061564 SRS20067085 GSM8004777 GSM8004777 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina NovaSeq 6000 SRX23112215 GSM8004778_r1 SRP482140 PRJNA1061564 SRS20067086 GSM8004778 GSM8004778 ChIP-Seq GENOMIC ChIP Cells were trypsinized and cross-linked with 1% formaldehyde (EMD Millipore) for 10 min at RT and quenched with 2 M glycine (Thermo Fisher Scientific) for 5 min at room temperature. Chromatin corresponding to 10 million cells for histone modifications was sheared with SFX250 Sonifier (Branson). The lysates were transferred to Diagenode tubes, sonicated 16 rounds of 30 seconds on 30 seconds off, vortexing every 4th round. Protein G beads (80uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting given Kacyl marks. 5 μg for each condition, at 4 °C overnight on a nutator. For the input sample, 200 μl of sheared nuclear lysate was removed and stored overnight at 4 °C. ChIP-DNA and input was reverse crosslinked by incubating overnight at 65 °C in 1% SDS, 1x TE buffer. On the third day, ChIP-DNA was treated with RNase A (Qiagen) and Proteinase K (ThermoFisher Scientific) and then purified using Qiagen QIAquick purification columns. The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated and libraries size-selected (200 - 400 bp) by gel extraction before PCR amplification. The purified libraries were subjected to paired-end sequencing on the Illumina HiSeq 4000/Novaseq 6000 SP to obtain an average of approximately 30 - 35 million uniquely mapped reads for each sample Illumina HiSeq 4000