SRX23130128 MCP2 SRP482457 bp0 Following the fusion primer methodology, universal forward primer 341f (5-CCTACGGGAGGCAGCAG-3) and universal reverse primer 515r (5-TATTACCGCGGCTGCTGG-3), were used to amplify the V3 region of 16S rRNA genes from each of the eight samples (Roy et al. 2020a; 2020b). Prior to sequencing, the amplified PCR products were quantified with a Qubitfluorometer (Thermo Fisher Scientific, USA) and then sequenced on an Ion S5 next-generation DNA sequencing machine (Thermo Fisher Scientific) SRS20082185 Posterior gut of mrigle MCP2 AMPLICON METAGENOMIC PCR Ion Torrent S5