SRX23131986 GSM8006367_r1 SRP482494 PRJNA1062393 SRS20083296 GSM8006367 GSM8006367 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina NovaSeq 6000 SRX23131987 GSM8006368_r1 SRP482494 PRJNA1062393 SRS20083297 GSM8006368 GSM8006368 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina NovaSeq 6000 SRX23131988 GSM8006369_r1 SRP482494 PRJNA1062393 SRS20083298 GSM8006369 GSM8006369 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina NovaSeq 6000 SRX23131989 GSM8006370_r1 SRP482494 PRJNA1062393 SRS20083299 GSM8006370 GSM8006370 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina NovaSeq 6000 SRX23131990 GSM8006371_r1 SRP482494 PRJNA1062393 SRS20083300 GSM8006371 GSM8006371 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131991 GSM8006372_r1 SRP482494 PRJNA1062393 SRS20083301 GSM8006372 GSM8006372 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131992 GSM8006373_r1 SRP482494 PRJNA1062393 SRS20083302 GSM8006373 GSM8006373 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131993 GSM8006374_r1 SRP482494 PRJNA1062393 SRS20083303 GSM8006374 GSM8006374 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131994 GSM8006375_r1 SRP482494 PRJNA1062393 SRS20083304 GSM8006375 GSM8006375 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131995 GSM8006376_r1 SRP482494 PRJNA1062393 SRS20083307 GSM8006376 GSM8006376 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131996 GSM8006377_r1 SRP482494 PRJNA1062393 SRS20083305 GSM8006377 GSM8006377 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000 SRX23131997 GSM8006378_r1 SRP482494 PRJNA1062393 SRS20083306 GSM8006378 GSM8006378 OTHER GENOMIC other CUT&Tag was performed using CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170). ~500,000 viable nuclei (30 mg tissue) were used per reaction. Tissue samples were Dounced 20 times with a loose pestle and 10 times with a tight pestle. 5 μg of undiluted primary antibody was added to each sample for an overnight incubation at 4C with orbital mixing. 100 μL of 1:100 of Guinea Pig Anti-Rabbit secondary antibody was then added to each reaction and the samples were incubated on an orbital rotator for 60 min at RT. Assembled pA-Tn5 Transposomes were added to each sample and reactions were incubated on an orbital rotator for 60 min at RT. 125 μL of Tagmentation Buffer (5 mL Dig-300 buffer and 50 µL 1 M MgCl2) was added to each sample and reactions were incubated at 37C for 1 h. Nextera i5 and i7 indexing primers were used and final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013). Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) CUT&Tag-ITTM assay kits (Active Motif # 53160, 53170) and Hatice S. Kaya-Okur et al., (Nat Comm 2019) Illumina HiSeq 4000