SRX23144160 JC1 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095080 JC1 JC1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144161 JC2 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095081 JC2 JC2 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144162 XG2 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095082 XG2 XG2 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144163 XG3 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095083 XG3 XG3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144164 XY1 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095084 XY1 XY1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144165 XY2 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095085 XY2 XY2 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144166 XY3 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095086 XY3 XY3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144167 JC3 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095087 JC3 JC3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144168 GC1 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095088 GC1 GC1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144169 GC2 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095089 GC2 GC2 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144170 GC3 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095090 GC3 GC3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144171 WS1 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095091 WS1 WS1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144172 WS2 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095092 WS2 WS2 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144173 WS3 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095094 WS3 WS3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX23144174 XG1 SRP482712 bp0 Bletilla striata, a perennial herbaceous plant belonging to the Orchidaceae family, is notable for its significant medicinal value. The growth, development, and chemical composition of this plant are profoundly influenced by environmental factors. However, there is a notable gap in multidimensional research investigating the interrelations between the key chemical components of Bletilla striata, micro-level microbial factors, and macro-level climatic elements. This study utilized high-throughput sequencing technology to analyze the bacterial and fungal community composition in the rhizosphere soil of Bletilla striata cultivation sites in Dali Prefecture. The physicochemical properties of the soil and the concentration of major active components in Bletilla striata medicinal materials were measured. Furthermore, the study examined the correlations between soil microbial diversity, physicochemical characteristics, and the quality of the medicinal materials. The results indicated that Proteobacteria, Acidobacteriota, and Bacteroidota were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the predominant fungal phyla. The composition of bacterial and fungal communities was closely related to the physicochemical properties of the soil (organic matter, nitrogen content, available potassium) and climatic factors (latitude and highest average temperature). The concentrations of medicinal active components in Bletilla striata showed a positive correlation with Actinobacteria and Bacteroidota, and a negative correlation with Acidobacteriota and Ascomycota. Additionally, the symbiotic network analysis of bacteria and fungi revealed regional differences in microbial interaction patterns among different production areas, reflecting the complex interdependencies among microbes. Overall, there is a significant association between soil microbes, environmental factors, and the quality of Bletilla striata medicinal materials. The composition and function of soil microbial communities may be key factors influencing the formation of medicinal components in Bletilla striata. These findings provide important ecological information for optimizing the cultivation environment of Bletilla striata and improving the quality of medicinal materials. SRS20095093 XG1 XG1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000