SRX23174298 Control 1 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123594 Control-119512 Control 1 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174299 Control 2 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123595 Control-119513 Control 2 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174300 Grin1KD 2 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123596 Grin1KD-119515 Grin1KD 2 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174301 Grin1KD 3 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123598 Grin1KD-119516 Grin1KD 3 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174302 Grin1KD 4 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123597 Grin1KD-149742 Grin1KD 4 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174303 Grin1KD 5 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123599 Grin1KD-153307 Grin1KD 5 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174304 Grin1KD 6 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123600 Grin1KD-153308 Grin1KD 6 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174305 Grin1KD 7 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123601 Grin1KD-153316 Grin1KD 7 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174306 Control 3 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123602 Control-149739 Control 3 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174307 Control 4 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123603 Control-149740 Control 4 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174308 Control 5 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123604 Control-149741 Control 5 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174309 Control 6 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123605 Control-153309 Control 6 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174310 Control 7 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123606 Control-153310 Control 7 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174311 Control 8 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123607 Control-153312 Control 8 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174312 Control 9 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123609 Control-153313 Control 9 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500 SRX23174313 Grin1KD 1 SRP483350 bp0 Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection. Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed. SRS20123608 Grin1KD-119514 Grin1KD 1 RNA-Seq TRANSCRIPTOMIC PolyA NextSeq 500