SRX23214294 GSM8016684_r1 SRP483677 PRJNA1064600 SRS20137814 GSM8016684 GSM8016684 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214295 GSM8016685_r1 SRP483677 PRJNA1064600 SRS20137815 GSM8016685 GSM8016685 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214296 GSM8016686_r1 SRP483677 PRJNA1064600 SRS20137816 GSM8016686 GSM8016686 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214297 GSM8016687_r1 SRP483677 PRJNA1064600 SRS20137817 GSM8016687 GSM8016687 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214298 GSM8016688_r1 SRP483677 PRJNA1064600 SRS20137818 GSM8016688 GSM8016688 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214299 GSM8016689_r1 SRP483677 PRJNA1064600 SRS20137819 GSM8016689 GSM8016689 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214300 GSM8016690_r1 SRP483677 PRJNA1064600 SRS20137820 GSM8016690 GSM8016690 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214301 GSM8016691_r1 SRP483677 PRJNA1064600 SRS20137821 GSM8016691 GSM8016691 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214302 GSM8016692_r1 SRP483677 PRJNA1064600 SRS20137822 GSM8016692 GSM8016692 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214303 GSM8016693_r1 SRP483677 PRJNA1064600 SRS20137823 GSM8016693 GSM8016693 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214304 GSM8016694_r1 SRP483677 PRJNA1064600 SRS20137824 GSM8016694 GSM8016694 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214305 GSM8016695_r1 SRP483677 PRJNA1064600 SRS20137825 GSM8016695 GSM8016695 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214306 GSM8016696_r1 SRP483677 PRJNA1064600 SRS20137827 GSM8016696 GSM8016696 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214307 GSM8016697_r1 SRP483677 PRJNA1064600 SRS20137826 GSM8016697 GSM8016697 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214308 GSM8016698_r1 SRP483677 PRJNA1064600 SRS20137828 GSM8016698 GSM8016698 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214309 GSM8016699_r1 SRP483677 PRJNA1064600 SRS20137829 GSM8016699 GSM8016699 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214310 GSM8016700_r1 SRP483677 PRJNA1064600 SRS20137830 GSM8016700 GSM8016700 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214311 GSM8016701_r1 SRP483677 PRJNA1064600 SRS20137831 GSM8016701 GSM8016701 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214312 GSM8016702_r1 SRP483677 PRJNA1064600 SRS20137832 GSM8016702 GSM8016702 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214313 GSM8016703_r1 SRP483677 PRJNA1064600 SRS20137833 GSM8016703 GSM8016703 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214314 GSM8016704_r1 SRP483677 PRJNA1064600 SRS20137834 GSM8016704 GSM8016704 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214315 GSM8016705_r1 SRP483677 PRJNA1064600 SRS20137835 GSM8016705 GSM8016705 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214316 GSM8016706_r1 SRP483677 PRJNA1064600 SRS20137836 GSM8016706 GSM8016706 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214317 GSM8016707_r1 SRP483677 PRJNA1064600 SRS20137837 GSM8016707 GSM8016707 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214318 GSM8016708_r1 SRP483677 PRJNA1064600 SRS20137838 GSM8016708 GSM8016708 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214319 GSM8016709_r1 SRP483677 PRJNA1064600 SRS20137839 GSM8016709 GSM8016709 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214320 GSM8016710_r1 SRP483677 PRJNA1064600 SRS20137840 GSM8016710 GSM8016710 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000 SRX23214321 GSM8016711_r1 SRP483677 PRJNA1064600 SRS20137841 GSM8016711 GSM8016711 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from cardiac RV and LV tissue using the RNeasy Mini or Universal Mini Kit (Qiagen Sciences, 74106 and 73404), cDNA synthesized (Invitrogen SuperScript III First-Strand Synthesis SuperMix for RT) and analyzed by qPCR using Taqman probes to human PKP2 or mouse Pkp2 gene, heart failure genes (Nppa, Nppb), and fibrosis genes (Col1a1, Col3a1, and Timp1). Mouse Gapdh served as an internal housekeeping gene control. Absolute transgene mRNA copy number was determined by RT-qPCR using a WPRE-specific RNA standard (GenScript) across six orders of magnitude. DSP protein level in both supernatant and pellet was analyzed to ensure a complete evaluation. From each replicate, 100 ng total RNA was extracted via the polyA-tail-specific protocol according to Illumina Inc.. RNA quality control was performed before library prep using Agilent TapeStation instrument. The RNA libraries were prepared using a Stranded Total RNA Library Prep with Ribo-Zero Plus kit (Illumina), which also removes ribosomal RNA. The libraries were sequenced as 2x50 base pair paired-end reads using Illumina NovaSeq 6000 using V1.5 reagent kit on S1 flow cell with an average of 25.66 million reads per each read file (51.32M reads per sample). Illumina NovaSeq 6000