<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT accession="SRX23223715" alias="GSM8017711_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223715</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017711_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017711: Control 'Tarocco' Blood orange juice sac, 0 days after storage, Biol rep 1; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146925">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146925</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017711</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017711</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223716" alias="GSM8017712_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223716</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017712_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017712: Control 'Tarocco' Blood orange juice sac, 0 days after storage, Biol rep 2; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146926">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146926</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017712</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017712</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223717" alias="GSM8017713_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223717</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017713_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017713: Control 'Tarocco' Blood orange juice sac, 0 days after storage, Biol rep 3; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146927">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146927</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017713</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017713</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223718" alias="GSM8017714_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223718</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017714_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017714: Control 'Tarocco' Blood orange juice sac, 30 days after storage, Biol rep 1; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146928">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146928</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017714</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017714</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223719" alias="GSM8017715_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223719</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017715_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017715: Control 'Tarocco' Blood orange juice sac, 30 days after storage, Biol rep 2; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146929">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146929</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017715</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017715</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223720" alias="GSM8017716_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223720</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017716_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017716: Control 'Tarocco' Blood orange juice sac, 30 days after storage, Biol rep 3; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146930">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146930</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017716</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017716</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223721" alias="GSM8017717_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223721</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017717_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017717: Control 'Tarocco' Blood orange juice sac, 50 days after storage, Biol rep 1; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146931">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146931</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017717</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017717</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223722" alias="GSM8017718_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223722</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017718_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017718: Control 'Tarocco' Blood orange juice sac, 50 days after storage, Biol rep 2; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146932">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146932</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017718</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017718</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223723" alias="GSM8017719_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223723</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017719_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017719: Control 'Tarocco' Blood orange juice sac, 50 days after storage, Biol rep 3; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146933">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146933</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017719</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017719</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223724" alias="GSM8017720_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223724</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017720_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017720: Drought treated 'Tarocco' Blood orange juice sac, 0 days after storage, Biol rep 1; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146934">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146934</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017720</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017720</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223725" alias="GSM8017721_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223725</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017721_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017721: Drought treated 'Tarocco' Blood orange juice sac, 0 days after storage, Biol rep 2; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146935">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146935</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017721</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017721</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223726" alias="GSM8017722_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223726</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017722_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017722: Drought treated 'Tarocco' Blood orange juice sac, 0 days after storage, Biol rep 3; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146936">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146936</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017722</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017722</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223727" alias="GSM8017723_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223727</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017723_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017723: Drought treated 'Tarocco' Blood orange juice sac, 30 days after storage, Biol rep 1; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146937">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146937</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017723</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017723</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223728" alias="GSM8017724_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223728</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017724_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017724: Drought treated 'Tarocco' Blood orange juice sac, 30 days after storage, Biol rep 2; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146938">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146938</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017724</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017724</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223729" alias="GSM8017725_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223729</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017725_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017725: Drought treated 'Tarocco' Blood orange juice sac, 30 days after storage, Biol rep 3; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146939">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146939</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017725</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017725</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223730" alias="GSM8017726_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223730</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017726_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017726: Drought treated 'Tarocco' Blood orange juice sac, 50 days after storage, Biol rep 1; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146940">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146940</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017726</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017726</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223731" alias="GSM8017727_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223731</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017727_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017727: Drought treated 'Tarocco' Blood orange juice sac, 50 days after storage, Biol rep 2; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146941">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146941</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017727</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017727</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX23223732" alias="GSM8017728_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX23223732</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM8017728_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM8017728: Drought treated 'Tarocco' Blood orange juice sac, 50 days after storage, Biol rep 3; Citrus sinensis; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP483818">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP483818</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA1064964</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS20146942">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS20146942</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM8017728</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM8017728</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number &gt;7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
</EXPERIMENT_SET>
