SRX23225235 POTs-G00 SRP436466 PRJNA967093 Targeted amplicons of potential off-target sites in strain G00 were sent to GENEWIZ for library construction and sequencing SRS17585046 SAMN34578253 POTs-G00 AMPLICON GENOMIC PCR Illumina NovaSeq 6000 SRX23225236 POTs-1a SRP436466 PRJNA967093 Targeted amplicons of potential off-target sites in strain G00 treated by pWLT-duBE-1a-an5C were sent to GENEWIZ for library construction and sequencing SRS17585047 SAMN34578254 POTs-1a AMPLICON GENOMIC PCR Illumina NovaSeq 6000 SRX23225237 POTs-1a-Sp4 SRP436466 PRJNA967093 Targeted amplicons of potential off-target sites in strain G00 treated by pWLT-duBE-1a-an5C-Sp4 were sent to GENEWIZ for library construction and sequencing SRS17585048 SAMN34578255 POTs-1a-Sp4 AMPLICON GENOMIC PCR Illumina NovaSeq 6000 SRX23225238 POTs-2b SRP436466 PRJNA967093 Targeted amplicons of potential off-target sites in strain G00 treated by pWLT-duBE-2b-an5C were sent to GENEWIZ for library construction and sequencing SRS17585049 SAMN34578256 POTs-2b AMPLICON GENOMIC PCR Illumina NovaSeq 6000 SRX23225239 POTs-2b-Sp4 SRP436466 PRJNA967093 Targeted amplicons of potential off-target sites in strain G00 treated by pWLT-duBE-2b-an5C-Sp4 were sent to GENEWIZ for library construction and sequencing SRS17585050 SAMN34578257 POTs-2b-Sp4 AMPLICON GENOMIC PCR Illumina NovaSeq 6000