SRX23306106 GSM8024339_r1 SRP484583 PRJNA1066372 SRS20182795 GSM8024339 GSM8024339 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306107 GSM8024340_r1 SRP484583 PRJNA1066372 SRS20182796 GSM8024340 GSM8024340 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306108 GSM8024341_r1 SRP484583 PRJNA1066372 SRS20182797 GSM8024341 GSM8024341 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306109 GSM8024342_r1 SRP484583 PRJNA1066372 SRS20182798 GSM8024342 GSM8024342 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306110 GSM8024343_r1 SRP484583 PRJNA1066372 SRS20182799 GSM8024343 GSM8024343 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306111 GSM8024344_r1 SRP484583 PRJNA1066372 SRS20182800 GSM8024344 GSM8024344 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306112 GSM8024345_r1 SRP484583 PRJNA1066372 SRS20182801 GSM8024345 GSM8024345 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000 SRX23306113 GSM8024346_r1 SRP484583 PRJNA1066372 SRS20182802 GSM8024346 GSM8024346 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Tissue samples from 5 mice (2 mice from GC group, 3 mice from GC+ICB group) were processed with mechanical chopping with blades, then disociated with Collagenase Type I (Worthington), Hyaluronidase (Sigma H3506), and DNase I (Sigma DN25). The cell suspension was passed through a 70-um filter, followed by 1×RBC lysis buffer treatment. A magnetically activated cell sorting (MACS) column was used to obtain CD45+ and CD45– cells from each tumor sample, and then the cells were mixed at a ratio of 1:1 and captured using the 10x Chromium controller. Single cells were processed with the Chromium Controller Single Cell Platform using the Gel Bead, Chip and Libraray Kits (10x Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, samples were processed using kits pertaining to the Chromium Next GEM Single Cell 3' Kit v3.1 barcoding chemistry of 10x Genomics. Libraries were constructed according to the standard 10x Genomics protocol (Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols, and Single Cell 3' Reagent Kits v3.1 (dual index) User Guide) with dual index and performed the paired-end sequencing on NextSeq 2000 (llumina, San Diego, CA). The same library was sequenced across two flowcells, with two lanes per flowcell (4 lanes in total). NextSeq 2000