SRX23307098 GSM8023252_r1 SRP484642 PRJNA1066286 SRS20183678 GSM8023252 GSM8023252 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Patient tumor tissue were dissociated using collagenase and DNase. Patient-derived PCO, and mixed PCO-CAF were dissociated using TrypLE™ Express (Thermo Fisher Scientific). Then, scRNA were performed after cell counting and viability analysis. Single-cell RNA sequencing was performed by using the Chromium Next GEM Single Cell 3' Reagent Kits (10x Genomics Inc., Pleasanton, CA, USA) and was sequenced on a Novaseq 6000 platform according to the manufacturer's instructions Illumina NovaSeq 6000 SRX23307099 GSM8023254_r1 SRP484642 PRJNA1066286 SRS20183679 GSM8023254 GSM8023254 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Patient tumor tissue were dissociated using collagenase and DNase. Patient-derived PCO, and mixed PCO-CAF were dissociated using TrypLE™ Express (Thermo Fisher Scientific). Then, scRNA were performed after cell counting and viability analysis. Single-cell RNA sequencing was performed by using the Chromium Next GEM Single Cell 3' Reagent Kits (10x Genomics Inc., Pleasanton, CA, USA) and was sequenced on a Novaseq 6000 platform according to the manufacturer's instructions Illumina NovaSeq 6000 SRX23307100 GSM8023256_r1 SRP484642 PRJNA1066286 SRS20183677 GSM8023256 GSM8023256 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Patient tumor tissue were dissociated using collagenase and DNase. Patient-derived PCO, and mixed PCO-CAF were dissociated using TrypLE™ Express (Thermo Fisher Scientific). Then, scRNA were performed after cell counting and viability analysis. Single-cell RNA sequencing was performed by using the Chromium Next GEM Single Cell 3' Reagent Kits (10x Genomics Inc., Pleasanton, CA, USA) and was sequenced on a Novaseq 6000 platform according to the manufacturer's instructions Illumina NovaSeq 6000