SRX23336829 STU-SP2022 Nanopore sequencing SRP484113 PRJNA1059155 Approximately 8-10 g of genomic DNA (> 50 kb) was selected using the SageHLS HMW library system (Sage Science, Beverley MA, USA) and processed w5000 with the Ligation Sequencing 1D Kit (Catalog No. SQK-LSK109, Oxford Nanopore Technologies, Shanghai, China) following the manufacturer's instructions. Library construction and sequencing were conducted on the PromethION (Oxford Nanopore Technologies) at the Genome Center of Grandomics (Wuhan, China). After quality inspection, large DNA fragments were recovered using the BluePippin automatic nucleic acid recovery instrument. Terminal repair, A-tailing, and ligation were performed using the LSK109 connection kit. Qubit was employed to assess the constructed DNA library precisely. The library was loaded into a flow cell, transferred to the PromethION sequencer, and subjected to real-time single-molecule sequencing. SRS20157316 SAMN39200060 STU-SP2022 Nanopore sequencing WGS GENOMIC RANDOM PromethION