SRX23376771 Fecal_DNA_002 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238710 FDNA_002 Fecal_DNA_002 OTHER METAGENOMIC PCR MinION SRX23376772 Fecal_DNA_004 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238712 FDNA_004 Fecal_DNA_004 OTHER METAGENOMIC PCR MinION SRX23376773 Fecal_DNA_018 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238711 FDNA_018 Fecal_DNA_018 OTHER METAGENOMIC PCR MinION SRX23376774 Fecal_DNA_115 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238713 FDNA_115 Fecal_DNA_115 OTHER METAGENOMIC PCR MinION SRX23376775 Fecal_DNA_116 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238714 FDNA_116 Fecal_DNA_116 OTHER METAGENOMIC PCR MinION SRX23376776 Fecal_DNA_117 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238715 FDNA_117 Fecal_DNA_117 OTHER METAGENOMIC PCR MinION SRX23376777 Fecal_DNA_118 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238716 FDNA_118 Fecal_DNA_118 OTHER METAGENOMIC PCR MinION SRX23376778 Fecal_DNA_119 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238717 FDNA_119 Fecal_DNA_119 OTHER METAGENOMIC PCR MinION SRX23376779 Fecal_DNA_120 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238718 FDNA_120 Fecal_DNA_120 OTHER METAGENOMIC PCR MinION SRX23376780 Fecal_DNA_121 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238719 FDNA_121 Fecal_DNA_121 OTHER METAGENOMIC PCR MinION SRX23376781 Fecal_DNA_122 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238720 FDNA_122 Fecal_DNA_122 OTHER METAGENOMIC PCR MinION SRX23376782 Fecal_DNA_123 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238721 FDNA_123 Fecal_DNA_123 OTHER METAGENOMIC PCR MinION SRX23376783 Fecal_DNA_124 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238722 FDNA_124 Fecal_DNA_124 OTHER METAGENOMIC PCR MinION SRX23376784 Fecal_DNA_019 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238723 FDNA_019 Fecal_DNA_019 OTHER METAGENOMIC PCR MinION SRX23376785 Fecal_DNA_125 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238725 FDNA_125 Fecal_DNA_125 OTHER METAGENOMIC PCR MinION SRX23376786 Fecal_DNA_126 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238724 FDNA_126 Fecal_DNA_126 OTHER METAGENOMIC PCR MinION SRX23376787 Fecal_DNA_127 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238726 FDNA_127 Fecal_DNA_127 OTHER METAGENOMIC PCR MinION SRX23376788 Fecal_DNA_128 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238727 FDNA_128 Fecal_DNA_128 OTHER METAGENOMIC PCR MinION SRX23376789 Fecal_DNA_129 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238728 FDNA_129 Fecal_DNA_129 OTHER METAGENOMIC PCR MinION SRX23376790 Fecal_DNA_130 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238729 FDNA_130 Fecal_DNA_130 OTHER METAGENOMIC PCR MinION SRX23376791 Fecal_DNA_131 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238731 FDNA_131 Fecal_DNA_131 OTHER METAGENOMIC PCR MinION SRX23376792 Fecal_DNA_132 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238730 FDNA_132 Fecal_DNA_132 OTHER METAGENOMIC PCR MinION SRX23376793 Fecal_DNA_134 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238732 FDNA_134 Fecal_DNA_134 OTHER METAGENOMIC PCR MinION SRX23376794 Fecal_DNA_135 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238733 FDNA_135 Fecal_DNA_135 OTHER METAGENOMIC PCR MinION SRX23376795 Fecal_DNA_020 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238734 FDNA_020 Fecal_DNA_020 OTHER METAGENOMIC PCR MinION SRX23376796 Fecal_DNA_136 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238735 FDNA_136 Fecal_DNA_136 OTHER METAGENOMIC PCR MinION SRX23376797 Fecal_DNA_137 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238736 FDNA_137 Fecal_DNA_137 OTHER METAGENOMIC PCR MinION SRX23376798 Fecal_DNA_138 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238737 FDNA_138 Fecal_DNA_138 OTHER METAGENOMIC PCR MinION SRX23376799 Fecal_DNA_139 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238739 FDNA_139 Fecal_DNA_139 OTHER METAGENOMIC PCR MinION SRX23376800 Fecal_DNA_140 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238738 FDNA_140 Fecal_DNA_140 OTHER METAGENOMIC PCR MinION SRX23376801 Fecal_DNA_141 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238741 FDNA_141 Fecal_DNA_141 OTHER METAGENOMIC PCR MinION SRX23376802 Fecal_DNA_142 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238740 FDNA_142 Fecal_DNA_142 OTHER METAGENOMIC PCR MinION SRX23376803 Fecal_DNA_143 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238742 FDNA_143 Fecal_DNA_143 OTHER METAGENOMIC PCR MinION SRX23376804 Fecal_DNA_144 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238743 FDNA_144 Fecal_DNA_144 OTHER METAGENOMIC PCR MinION SRX23376805 Fecal_DNA_145 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238745 FDNA_145 Fecal_DNA_145 OTHER METAGENOMIC PCR MinION SRX23376806 Fecal_DNA_021 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238744 FDNA_021 Fecal_DNA_021 OTHER METAGENOMIC PCR MinION SRX23376807 Fecal_DNA_146 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238746 FDNA_146 Fecal_DNA_146 OTHER METAGENOMIC PCR MinION SRX23376808 Fecal_DNA_147 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238747 FDNA_147 Fecal_DNA_147 OTHER METAGENOMIC PCR MinION SRX23376809 Fecal_DNA_148 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238749 FDNA_148 Fecal_DNA_148 OTHER METAGENOMIC PCR MinION SRX23376810 Fecal_DNA_149 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238748 FDNA_149 Fecal_DNA_149 OTHER METAGENOMIC PCR MinION SRX23376811 Fecal_DNA_150 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238750 FDNA_150 Fecal_DNA_150 OTHER METAGENOMIC PCR MinION SRX23376812 Fecal_DNA_151 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238751 FDNA_151 Fecal_DNA_151 OTHER METAGENOMIC PCR MinION SRX23376813 Fecal_DNA_152 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238752 FDNA_152 Fecal_DNA_152 OTHER METAGENOMIC PCR MinION SRX23376814 Fecal_DNA_153 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238753 FDNA_153 Fecal_DNA_153 OTHER METAGENOMIC PCR MinION SRX23376815 Fecal_DNA_154 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238754 FDNA_154 Fecal_DNA_154 OTHER METAGENOMIC PCR MinION SRX23376816 Fecal_DNA_155 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238755 FDNA_155 Fecal_DNA_155 OTHER METAGENOMIC PCR MinION SRX23376817 Fecal_DNA_022 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238756 FDNA_022 Fecal_DNA_022 OTHER METAGENOMIC PCR MinION SRX23376818 Fecal_DNA_156 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238757 FDNA_156 Fecal_DNA_156 OTHER METAGENOMIC PCR MinION SRX23376819 Fecal_DNA_173 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238760 FDNA_173 Fecal_DNA_173 OTHER METAGENOMIC PCR MinION SRX23376820 Fecal_DNA_174 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238759 FDNA_174 Fecal_DNA_174 OTHER METAGENOMIC PCR MinION SRX23376821 Fecal_DNA_175 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238758 FDNA_175 Fecal_DNA_175 OTHER METAGENOMIC PCR MinION SRX23376822 Fecal_DNA_178 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238761 FDNA_178 Fecal_DNA_178 OTHER METAGENOMIC PCR MinION SRX23376823 Fecal_DNA_024 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238762 FDNA_024 Fecal_DNA_024 OTHER METAGENOMIC PCR MinION SRX23376824 Fecal_DNA_025 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238763 FDNA_025 Fecal_DNA_025 OTHER METAGENOMIC PCR MinION SRX23376825 Fecal_DNA_026 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238764 FDNA_026 Fecal_DNA_026 OTHER METAGENOMIC PCR MinION SRX23376826 Fecal_DNA_027 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238765 FDNA_027 Fecal_DNA_027 OTHER METAGENOMIC PCR MinION SRX23376827 Fecal_DNA_005 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238767 FDNA_005 Fecal_DNA_005 OTHER METAGENOMIC PCR MinION SRX23376828 Fecal_DNA_028 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238766 FDNA_028 Fecal_DNA_028 OTHER METAGENOMIC PCR MinION SRX23376829 Fecal_DNA_029 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238768 FDNA_029 Fecal_DNA_029 OTHER METAGENOMIC PCR MinION SRX23376830 Fecal_DNA_030 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238769 FDNA_030 Fecal_DNA_030 OTHER METAGENOMIC PCR MinION SRX23376831 Fecal_DNA_031 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238770 FDNA_031 Fecal_DNA_031 OTHER METAGENOMIC PCR MinION SRX23376832 Fecal_DNA_032 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238771 FDNA_032 Fecal_DNA_032 OTHER METAGENOMIC PCR MinION SRX23376833 Fecal_DNA_033 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238772 FDNA_033 Fecal_DNA_033 OTHER METAGENOMIC PCR MinION SRX23376834 Fecal_DNA_034 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238773 FDNA_034 Fecal_DNA_034 OTHER METAGENOMIC PCR MinION SRX23376835 Fecal_DNA_049 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238775 FDNA_049 Fecal_DNA_049 OTHER METAGENOMIC PCR MinION SRX23376836 Fecal_DNA_050 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238774 FDNA_050 Fecal_DNA_050 OTHER METAGENOMIC PCR MinION SRX23376837 Fecal_DNA_051 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238776 FDNA_051 Fecal_DNA_051 OTHER METAGENOMIC PCR MinION SRX23376838 Fecal_DNA_052 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238778 FDNA_052 Fecal_DNA_052 OTHER METAGENOMIC PCR MinION SRX23376839 Fecal_DNA_053 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238777 FDNA_053 Fecal_DNA_053 OTHER METAGENOMIC PCR MinION SRX23376840 Fecal_DNA_054 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238779 FDNA_054 Fecal_DNA_054 OTHER METAGENOMIC PCR MinION SRX23376841 Fecal_DNA_055 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238780 FDNA_055 Fecal_DNA_055 OTHER METAGENOMIC PCR MinION SRX23376842 Fecal_DNA_056 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238781 FDNA_056 Fecal_DNA_056 OTHER METAGENOMIC PCR MinION SRX23376843 Fecal_DNA_057 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238782 FDNA_057 Fecal_DNA_057 OTHER METAGENOMIC PCR MinION SRX23376844 Fecal_DNA_011 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238783 FDNA_011 Fecal_DNA_011 OTHER METAGENOMIC PCR MinION SRX23376845 Fecal_DNA_058 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238784 FDNA_058 Fecal_DNA_058 OTHER METAGENOMIC PCR MinION SRX23376846 Fecal_DNA_059 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238785 FDNA_059 Fecal_DNA_059 OTHER METAGENOMIC PCR MinION SRX23376847 Fecal_DNA_060 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238786 FDNA_060 Fecal_DNA_060 OTHER METAGENOMIC PCR MinION SRX23376848 Fecal_DNA_061 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238787 FDNA_061 Fecal_DNA_061 OTHER METAGENOMIC PCR MinION SRX23376849 Fecal_DNA_062 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238788 FDNA_062 Fecal_DNA_062 OTHER METAGENOMIC PCR MinION SRX23376850 Fecal_DNA_063 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238789 FDNA_063 Fecal_DNA_063 OTHER METAGENOMIC PCR MinION SRX23376851 Fecal_DNA_080 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238790 FDNA_080 Fecal_DNA_080 OTHER METAGENOMIC PCR MinION SRX23376852 Fecal_DNA_081 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238792 FDNA_081 Fecal_DNA_081 OTHER METAGENOMIC PCR MinION SRX23376853 Fecal_DNA_083 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238791 FDNA_083 Fecal_DNA_083 OTHER METAGENOMIC PCR MinION SRX23376854 Fecal_DNA_084 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238793 FDNA_084 Fecal_DNA_084 OTHER METAGENOMIC PCR MinION SRX23376855 Fecal_DNA_085 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238794 FDNA_085 Fecal_DNA_085 OTHER METAGENOMIC PCR MinION SRX23376856 Fecal_DNA_086 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238795 FDNA_086 Fecal_DNA_086 OTHER METAGENOMIC PCR MinION SRX23376857 Fecal_DNA_087 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238796 FDNA_087 Fecal_DNA_087 OTHER METAGENOMIC PCR MinION SRX23376858 Fecal_DNA_088 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238797 FDNA_088 Fecal_DNA_088 OTHER METAGENOMIC PCR MinION SRX23376859 Fecal_DNA_089 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238798 FDNA_089 Fecal_DNA_089 OTHER METAGENOMIC PCR MinION SRX23376860 Fecal_DNA_090 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238799 FDNA_090 Fecal_DNA_090 OTHER METAGENOMIC PCR MinION SRX23376861 Fecal_DNA_016 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238800 FDNA_016 Fecal_DNA_016 OTHER METAGENOMIC PCR MinION SRX23376862 Fecal_DNA_091 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238801 FDNA_091 Fecal_DNA_091 OTHER METAGENOMIC PCR MinION SRX23376863 Fecal_DNA_092 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238802 FDNA_092 Fecal_DNA_092 OTHER METAGENOMIC PCR MinION SRX23376864 Fecal_DNA_093 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238803 FDNA_093 Fecal_DNA_093 OTHER METAGENOMIC PCR MinION SRX23376865 Fecal_DNA_094 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238804 FDNA_094 Fecal_DNA_094 OTHER METAGENOMIC PCR MinION SRX23376866 Fecal_DNA_095 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238805 FDNA_095 Fecal_DNA_095 OTHER METAGENOMIC PCR MinION SRX23376867 Fecal_DNA_157 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238806 FDNA_157 Fecal_DNA_157 OTHER METAGENOMIC PCR MinION SRX23376868 Fecal_DNA_158 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238807 FDNA_158 Fecal_DNA_158 OTHER METAGENOMIC PCR MinION SRX23376869 Fecal_DNA_159 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238809 FDNA_159 Fecal_DNA_159 OTHER METAGENOMIC PCR MinION SRX23376870 Fecal_DNA_160 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238808 FDNA_160 Fecal_DNA_160 OTHER METAGENOMIC PCR MinION SRX23376871 Fecal_DNA_161 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238811 FDNA_161 Fecal_DNA_161 OTHER METAGENOMIC PCR MinION SRX23376872 Fecal_DNA_162 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238810 FDNA_162 Fecal_DNA_162 OTHER METAGENOMIC PCR MinION SRX23376873 Fecal_DNA_164 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238812 FDNA_164 Fecal_DNA_164 OTHER METAGENOMIC PCR MinION SRX23376874 Fecal_DNA_165 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238814 FDNA_165 Fecal_DNA_165 OTHER METAGENOMIC PCR MinION SRX23376875 Fecal_DNA_166 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238813 FDNA_166 Fecal_DNA_166 OTHER METAGENOMIC PCR MinION SRX23376876 Fecal_DNA_023 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238815 FDNA_023 Fecal_DNA_023 OTHER METAGENOMIC PCR MinION SRX23376877 Fecal_DNA_167 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238816 FDNA_167 Fecal_DNA_167 OTHER METAGENOMIC PCR MinION SRX23376878 Fecal_DNA_168 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238817 FDNA_168 Fecal_DNA_168 OTHER METAGENOMIC PCR MinION SRX23376879 Fecal_DNA_169 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238819 FDNA_169 Fecal_DNA_169 OTHER METAGENOMIC PCR MinION SRX23376880 Fecal_DNA_170 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238818 FDNA_170 Fecal_DNA_170 OTHER METAGENOMIC PCR MinION SRX23376881 Fecal_DNA_171 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238820 FDNA_171 Fecal_DNA_171 OTHER METAGENOMIC PCR MinION SRX23376882 Fecal_DNA_172 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238821 FDNA_172 Fecal_DNA_172 OTHER METAGENOMIC PCR MinION SRX23376883 Fecal_DNA_035 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238822 FDNA_035 Fecal_DNA_035 OTHER METAGENOMIC PCR MinION SRX23376884 Fecal_DNA_036 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238823 FDNA_036 Fecal_DNA_036 OTHER METAGENOMIC PCR MinION SRX23376885 Fecal_DNA_037 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238824 FDNA_037 Fecal_DNA_037 OTHER METAGENOMIC PCR MinION SRX23376886 Fecal_DNA_006 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238825 FDNA_006 Fecal_DNA_006 OTHER METAGENOMIC PCR MinION SRX23376887 Fecal_DNA_038 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238826 FDNA_038 Fecal_DNA_038 OTHER METAGENOMIC PCR MinION SRX23376888 Fecal_DNA_039 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238827 FDNA_039 Fecal_DNA_039 OTHER METAGENOMIC PCR MinION SRX23376889 Fecal_DNA_040 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238828 FDNA_040 Fecal_DNA_040 OTHER METAGENOMIC PCR MinION SRX23376890 Fecal_DNA_041 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238829 FDNA_041 Fecal_DNA_041 OTHER METAGENOMIC PCR MinION SRX23376891 Fecal_DNA_042 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238832 FDNA_042 Fecal_DNA_042 OTHER METAGENOMIC PCR MinION SRX23376892 Fecal_DNA_043 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238830 FDNA_043 Fecal_DNA_043 OTHER METAGENOMIC PCR MinION SRX23376893 Fecal_DNA_044 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238831 FDNA_044 Fecal_DNA_044 OTHER METAGENOMIC PCR MinION SRX23376894 Fecal_DNA_045 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238834 FDNA_045 Fecal_DNA_045 OTHER METAGENOMIC PCR MinION SRX23376895 Fecal_DNA_046 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238833 FDNA_046 Fecal_DNA_046 OTHER METAGENOMIC PCR MinION SRX23376896 Fecal_DNA_047 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238835 FDNA_047 Fecal_DNA_047 OTHER METAGENOMIC PCR MinION SRX23376897 Fecal_DNA_010 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238836 FDNA_010 Fecal_DNA_010 OTHER METAGENOMIC PCR MinION SRX23376898 Fecal_DNA_048 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238837 FDNA_048 Fecal_DNA_048 OTHER METAGENOMIC PCR MinION SRX23376899 Fecal_DNA_064 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238839 FDNA_064 Fecal_DNA_064 OTHER METAGENOMIC PCR MinION SRX23376900 Fecal_DNA_065 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238838 FDNA_065 Fecal_DNA_065 OTHER METAGENOMIC PCR MinION SRX23376901 Fecal_DNA_066 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238840 FDNA_066 Fecal_DNA_066 OTHER METAGENOMIC PCR MinION SRX23376902 Fecal_DNA_067 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238841 FDNA_067 Fecal_DNA_067 OTHER METAGENOMIC PCR MinION SRX23376903 Fecal_DNA_012 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238842 FDNA_012 Fecal_DNA_012 OTHER METAGENOMIC PCR MinION SRX23376904 Fecal_DNA_068 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238843 FDNA_068 Fecal_DNA_068 OTHER METAGENOMIC PCR MinION SRX23376905 Fecal_DNA_069 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238845 FDNA_069 Fecal_DNA_069 OTHER METAGENOMIC PCR MinION SRX23376906 Fecal_DNA_070 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238844 FDNA_070 Fecal_DNA_070 OTHER METAGENOMIC PCR MinION SRX23376907 Fecal_DNA_071 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238846 FDNA_071 Fecal_DNA_071 OTHER METAGENOMIC PCR MinION SRX23376908 Fecal_DNA_074 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238847 FDNA_074 Fecal_DNA_074 OTHER METAGENOMIC PCR MinION SRX23376909 Fecal_DNA_075 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238848 FDNA_075 Fecal_DNA_075 OTHER METAGENOMIC PCR MinION SRX23376910 Fecal_DNA_076 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238850 FDNA_076 Fecal_DNA_076 OTHER METAGENOMIC PCR MinION SRX23376911 Fecal_DNA_077 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238849 FDNA_077 Fecal_DNA_077 OTHER METAGENOMIC PCR MinION SRX23376912 Fecal_DNA_078 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238851 FDNA_078 Fecal_DNA_078 OTHER METAGENOMIC PCR MinION SRX23376913 Fecal_DNA_079 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238852 FDNA_079 Fecal_DNA_079 OTHER METAGENOMIC PCR MinION SRX23376914 Fecal_DNA_013 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238854 FDNA_013 Fecal_DNA_013 OTHER METAGENOMIC PCR MinION SRX23376915 Fecal_DNA_096 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238853 FDNA_096 Fecal_DNA_096 OTHER METAGENOMIC PCR MinION SRX23376916 Fecal_DNA_097 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238855 FDNA_097 Fecal_DNA_097 OTHER METAGENOMIC PCR MinION SRX23376917 Fecal_DNA_100 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238856 FDNA_100 Fecal_DNA_100 OTHER METAGENOMIC PCR MinION SRX23376918 Fecal_DNA_101 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238857 FDNA_101 Fecal_DNA_101 OTHER METAGENOMIC PCR MinION SRX23376919 Fecal_DNA_102 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238859 FDNA_102 Fecal_DNA_102 OTHER METAGENOMIC PCR MinION SRX23376920 Fecal_DNA_017 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238858 FDNA_017 Fecal_DNA_017 OTHER METAGENOMIC PCR MinION SRX23376921 Fecal_DNA_103 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238860 FDNA_103 Fecal_DNA_103 OTHER METAGENOMIC PCR MinION SRX23376922 Fecal_DNA_105 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238862 FDNA_105 Fecal_DNA_105 OTHER METAGENOMIC PCR MinION SRX23376923 Fecal_DNA_106 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238861 FDNA_106 Fecal_DNA_106 OTHER METAGENOMIC PCR MinION SRX23376924 Fecal_DNA_107 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238864 FDNA_107 Fecal_DNA_107 OTHER METAGENOMIC PCR MinION SRX23376925 Fecal_DNA_108 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238863 FDNA_108 Fecal_DNA_108 OTHER METAGENOMIC PCR MinION SRX23376926 Fecal_DNA_109 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238866 FDNA_109 Fecal_DNA_109 OTHER METAGENOMIC PCR MinION SRX23376927 Fecal_DNA_110 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238867 FDNA_110 Fecal_DNA_110 OTHER METAGENOMIC PCR MinION SRX23376928 Fecal_DNA_112 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238865 FDNA_112 Fecal_DNA_112 OTHER METAGENOMIC PCR MinION SRX23376929 Fecal_DNA_113 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238868 FDNA_113 Fecal_DNA_113 OTHER METAGENOMIC PCR MinION SRX23376930 Fecal_DNA_114 SRP485633 bp0 DNA was extracted from fecal samples using a QIAamp PowerFecal Pro kit (Qiagen). The Oxford Nanopore Technologies (ONT) Rapid 16S Barcoding Kit (SQK-16S024; utilizing Kit 9 chemistry) was used to prepare barcoded amplicon libraries for sequencing. In brief: the full-length bacterial 16S rRNA gene region (1.6 kb) was amplified via PCR using specific primers and between 20-40 ng of DNA template, a long-range master mix, and sample-specific barcode identifier. PCR products were purified and prepared for sequencing through a series of magnetic bead wash steps. Barcoded samples were pooled (pools of 21-24 samples) with ONT rapid sequencing adapter mixture into a final library for sequencing. Pooled libraries were sequenced on FLO-MIN106 MinION flow cells utilizing R9 sequencing chemistry, run for 24 hours using the ONT MinKNOW GUI (v4.3.20). SRS20238869 FDNA_114 Fecal_DNA_114 OTHER METAGENOMIC PCR MinION