SRX23456644 Of1 SRP486978 PRJNA1071197 The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel. SRS20309641 SAMN39681366 Of1 miRNA-Seq TRANSCRIPTOMIC other Illumina NovaSeq 6000 SRX23456645 Of2 SRP486978 PRJNA1071197 The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel. SRS20309642 SAMN39681367 Of2 miRNA-Seq TRANSCRIPTOMIC other Illumina NovaSeq 6000 SRX23456646 Of3 SRP486978 PRJNA1071197 The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel. SRS20309643 SAMN39681368 Of3 miRNA-Seq TRANSCRIPTOMIC other Illumina NovaSeq 6000 SRX23456647 SM-Of1 SRP486978 PRJNA1071197 The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel. SRS20309644 SAMN39681369 SM-Of1 miRNA-Seq TRANSCRIPTOMIC other Illumina NovaSeq 6000 SRX23456648 SM-Of2 SRP486978 PRJNA1071197 The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel. SRS20309645 SAMN39681370 SM-Of2 miRNA-Seq TRANSCRIPTOMIC other Illumina NovaSeq 6000 SRX23456649 SM-Of3 SRP486978 PRJNA1071197 The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel. SRS20309646 SAMN39681371 SM-Of3 miRNA-Seq TRANSCRIPTOMIC other Illumina NovaSeq 6000