Plasmid sequencing of miniprepped, restriction digest linearized (SceI) : synthetic plsamid pool of barcodes and ORFs

SRX23528203 PacBio library, prepared without due respect Plasmid sequencing of miniprepped, restriction digest linearized (SceI) : synthetic plsamid pool of barcodes and ORFs SRP488028 bp0 Plasmids were miniprepped from E. coli using Qiagen miniprep kit, digested using I-SceI, observed to be linear and intact, then prepared for sequencing using PacBio ligation kit. Run on a Sequel 2 by UofArizona Genomics Core. Samples were apparenty not nick-repaired or treated with due respect, and reads appear truncated. SRS20377909 First_Plasmid_Pool PacBio library, prepared without due respect OTHER SYNTHETIC Restriction Digest PacBio RS II SRX23528204 Nanopore library prepared without due respect Plasmid sequencing of miniprepped, restriction digest linearized (SceI) : synthetic plsamid pool of barcodes and ORFs SRP488028 bp0 Plasmids were miniprepped from E. coli using Qiagen miniprep kit, digested using I-SceI, observed to be linear and intact, then prepared for sequencing using ONT Nanopore ligation kit. sequencing/sequencing_MIN112_DNA_SQK-Q20EA:FLO-MIN112:SQK-NBD112-24 This time I used nick-repaired or treated with due respect, and many fewer reads appear truncated. SRS20377908 Second_Plasmid_Pool Nanopore library prepared without due respect OTHER SYNTHETIC Restriction Digest MinION