SRP488029 PRJNA1072988 Mus musculus Genome sequencing and assembly This study aimed to examinate the potential effects of regular swimming exercise on mitigating the dysregulation observed in the aging lens of mice.Male C57BL/6J mice were categorized into two age groups: young(3 month old) and aging (18 month old). Young mice comprised young sedentary (YS) group, while aging mice were subsequently divided into aging sedentary (AS) group and aging exercising (AE) group. Mice in AE groups underwent a swimming regimen that consisted of adaptive swimming (5 min/d, 3d/wk for 1 week), stage-one swimming (10 min/d, 3d/wk for 4 week) and stage-two swimming (15 min/d, 3d/wk for 4 week). RNA-seq analysis was utilized to examine transcriptomic changes among the various groups.The analysis of the transcriptome between the YS and AS groups revealed a total of 92 differentially expressed genes (DEGs), of which 28 genes have been previously reported. GSEA analysis showed that our swimming exercise significantly downregulated approximately half of the pathways that undergo alterations during the aging process, where notable improvement was found in the categories of 'environmental information processing' including 'calcium signaling pathway', 'cell adhesion molecules' and 'neuroactive ligand receptor interaction'. Mus musculus