SRX23533203 GSM8062864_r1 SRP488113 PRJNA1073397 SRS20382201 GSM8062864 GSM8062864 RNA-Seq TRANSCRIPTOMIC cDNA Isolated total RNA from adult microglia was used for RNA-seq analysis. cDNA library construction and sequencing were performed by Jingjie PTM BioLab (Hangzhou, China) using the Illumina platform. High-quality reads were aligned to the mouse reference genome using Hisat2 (v.2.0.5). Each sample was sequenced with a data depth of 10G. Expression levels for each of the genes were quantified by stringtie (v.2.2.0). Differential gene analysis was carried out using the DESeq2 package (v.1.20.0). Differentially expressed genes (DEGs) with ≥2-fold change and P<0.05 were identified. Gene functional annotation overrepresented by DEGs was performed using the GO (Gene Ontology) database. The gene set enrichment analysis (GSEA) was performed using the ranked list of genes based on the log-fold change obtained from the differential gene analysis. RNA libraries were prepared for sequencing using standard Illumina protocols Illumina NovaSeq 6000 SRX23533204 GSM8062865_r1 SRP488113 PRJNA1073397 SRS20382202 GSM8062865 GSM8062865 RNA-Seq TRANSCRIPTOMIC cDNA Isolated total RNA from adult microglia was used for RNA-seq analysis. cDNA library construction and sequencing were performed by Jingjie PTM BioLab (Hangzhou, China) using the Illumina platform. High-quality reads were aligned to the mouse reference genome using Hisat2 (v.2.0.5). Each sample was sequenced with a data depth of 10G. Expression levels for each of the genes were quantified by stringtie (v.2.2.0). Differential gene analysis was carried out using the DESeq2 package (v.1.20.0). Differentially expressed genes (DEGs) with ≥2-fold change and P<0.05 were identified. Gene functional annotation overrepresented by DEGs was performed using the GO (Gene Ontology) database. The gene set enrichment analysis (GSEA) was performed using the ranked list of genes based on the log-fold change obtained from the differential gene analysis. RNA libraries were prepared for sequencing using standard Illumina protocols Illumina NovaSeq 6000 SRX23533205 GSM8062866_r1 SRP488113 PRJNA1073397 SRS20382203 GSM8062866 GSM8062866 RNA-Seq TRANSCRIPTOMIC cDNA Isolated total RNA from adult microglia was used for RNA-seq analysis. cDNA library construction and sequencing were performed by Jingjie PTM BioLab (Hangzhou, China) using the Illumina platform. High-quality reads were aligned to the mouse reference genome using Hisat2 (v.2.0.5). Each sample was sequenced with a data depth of 10G. Expression levels for each of the genes were quantified by stringtie (v.2.2.0). Differential gene analysis was carried out using the DESeq2 package (v.1.20.0). Differentially expressed genes (DEGs) with ≥2-fold change and P<0.05 were identified. Gene functional annotation overrepresented by DEGs was performed using the GO (Gene Ontology) database. The gene set enrichment analysis (GSEA) was performed using the ranked list of genes based on the log-fold change obtained from the differential gene analysis. RNA libraries were prepared for sequencing using standard Illumina protocols Illumina NovaSeq 6000 SRX23533206 GSM8062867_r1 SRP488113 PRJNA1073397 SRS20382204 GSM8062867 GSM8062867 RNA-Seq TRANSCRIPTOMIC cDNA Isolated total RNA from adult microglia was used for RNA-seq analysis. cDNA library construction and sequencing were performed by Jingjie PTM BioLab (Hangzhou, China) using the Illumina platform. High-quality reads were aligned to the mouse reference genome using Hisat2 (v.2.0.5). Each sample was sequenced with a data depth of 10G. Expression levels for each of the genes were quantified by stringtie (v.2.2.0). Differential gene analysis was carried out using the DESeq2 package (v.1.20.0). Differentially expressed genes (DEGs) with ≥2-fold change and P<0.05 were identified. Gene functional annotation overrepresented by DEGs was performed using the GO (Gene Ontology) database. The gene set enrichment analysis (GSEA) was performed using the ranked list of genes based on the log-fold change obtained from the differential gene analysis. RNA libraries were prepared for sequencing using standard Illumina protocols Illumina NovaSeq 6000 SRX23533207 GSM8062868_r1 SRP488113 PRJNA1073397 SRS20382205 GSM8062868 GSM8062868 RNA-Seq TRANSCRIPTOMIC cDNA Isolated total RNA from adult microglia was used for RNA-seq analysis. cDNA library construction and sequencing were performed by Jingjie PTM BioLab (Hangzhou, China) using the Illumina platform. High-quality reads were aligned to the mouse reference genome using Hisat2 (v.2.0.5). Each sample was sequenced with a data depth of 10G. Expression levels for each of the genes were quantified by stringtie (v.2.2.0). Differential gene analysis was carried out using the DESeq2 package (v.1.20.0). Differentially expressed genes (DEGs) with ≥2-fold change and P<0.05 were identified. Gene functional annotation overrepresented by DEGs was performed using the GO (Gene Ontology) database. The gene set enrichment analysis (GSEA) was performed using the ranked list of genes based on the log-fold change obtained from the differential gene analysis. RNA libraries were prepared for sequencing using standard Illumina protocols Illumina NovaSeq 6000 SRX23533208 GSM8062869_r1 SRP488113 PRJNA1073397 SRS20382206 GSM8062869 GSM8062869 RNA-Seq TRANSCRIPTOMIC cDNA Isolated total RNA from adult microglia was used for RNA-seq analysis. cDNA library construction and sequencing were performed by Jingjie PTM BioLab (Hangzhou, China) using the Illumina platform. High-quality reads were aligned to the mouse reference genome using Hisat2 (v.2.0.5). Each sample was sequenced with a data depth of 10G. Expression levels for each of the genes were quantified by stringtie (v.2.2.0). Differential gene analysis was carried out using the DESeq2 package (v.1.20.0). Differentially expressed genes (DEGs) with ≥2-fold change and P<0.05 were identified. Gene functional annotation overrepresented by DEGs was performed using the GO (Gene Ontology) database. The gene set enrichment analysis (GSEA) was performed using the ranked list of genes based on the log-fold change obtained from the differential gene analysis. RNA libraries were prepared for sequencing using standard Illumina protocols Illumina NovaSeq 6000