SRX23550070 GSM8065162_r1 SRP488466 PRJNA1073892 SRS20397299 GSM8065162 GSM8065162 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550071 GSM8065163_r1 SRP488466 PRJNA1073892 SRS20397301 GSM8065163 GSM8065163 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550072 GSM8065164_r1 SRP488466 PRJNA1073892 SRS20397311 GSM8065164 GSM8065164 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550073 GSM8065165_r1 SRP488466 PRJNA1073892 SRS20397302 GSM8065165 GSM8065165 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550074 GSM8065166_r1 SRP488466 PRJNA1073892 SRS20397300 GSM8065166 GSM8065166 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550075 GSM8065167_r1 SRP488466 PRJNA1073892 SRS20397306 GSM8065167 GSM8065167 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550076 GSM8065168_r1 SRP488466 PRJNA1073892 SRS20397307 GSM8065168 GSM8065168 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550077 GSM8065169_r1 SRP488466 PRJNA1073892 SRS20397305 GSM8065169 GSM8065169 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550078 GSM8065170_r1 SRP488466 PRJNA1073892 SRS20397304 GSM8065170 GSM8065170 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550079 GSM8065171_r1 SRP488466 PRJNA1073892 SRS20397303 GSM8065171 GSM8065171 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550080 GSM8065172_r1 SRP488466 PRJNA1073892 SRS20397310 GSM8065172 GSM8065172 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550081 GSM8065173_r1 SRP488466 PRJNA1073892 SRS20397312 GSM8065173 GSM8065173 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550082 GSM8065174_r1 SRP488466 PRJNA1073892 SRS20397308 GSM8065174 GSM8065174 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550083 GSM8065175_r1 SRP488466 PRJNA1073892 SRS20397309 GSM8065175 GSM8065175 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550084 GSM8065176_r1 SRP488466 PRJNA1073892 SRS20397313 GSM8065176 GSM8065176 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550085 GSM8065177_r1 SRP488466 PRJNA1073892 SRS20397315 GSM8065177 GSM8065177 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550086 GSM8065178_r1 SRP488466 PRJNA1073892 SRS20397331 GSM8065178 GSM8065178 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550087 GSM8065179_r1 SRP488466 PRJNA1073892 SRS20397314 GSM8065179 GSM8065179 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550088 GSM8065180_r1 SRP488466 PRJNA1073892 SRS20397329 GSM8065180 GSM8065180 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550089 GSM8065181_r1 SRP488466 PRJNA1073892 SRS20397317 GSM8065181 GSM8065181 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550090 GSM8065182_r1 SRP488466 PRJNA1073892 SRS20397330 GSM8065182 GSM8065182 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550091 GSM8065183_r1 SRP488466 PRJNA1073892 SRS20397332 GSM8065183 GSM8065183 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550092 GSM8065184_r1 SRP488466 PRJNA1073892 SRS20397333 GSM8065184 GSM8065184 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550093 GSM8065185_r1 SRP488466 PRJNA1073892 SRS20397321 GSM8065185 GSM8065185 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550094 GSM8065186_r1 SRP488466 PRJNA1073892 SRS20397334 GSM8065186 GSM8065186 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550095 GSM8065187_r1 SRP488466 PRJNA1073892 SRS20397318 GSM8065187 GSM8065187 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000 SRX23550096 GSM8065188_r1 SRP488466 PRJNA1073892 SRS20397336 GSM8065188 GSM8065188 RNA-Seq TRANSCRIPTOMIC cDNA Tregs were added to insert with inflamed (24h) monolayer of BBB-EC to interact and migrate accross BBB-EC for 24h. Tregs were also cultured without BBB-EC but in same medium for 24h. Next, cells were collected and mRNA was isolated. mRNA was harvested using Qiagen's RNeasy micro kit RNA libraries for RNA-seq were prepared using SMART-Seq® Stranded Kit Illumina NovaSeq 6000