SRX23729683 GSM8105694_r1 SRP491500 PRJNA1079999 SRS20558441 GSM8105694 GSM8105694 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729684 GSM8105695_r1 SRP491500 PRJNA1079999 SRS20558443 GSM8105695 GSM8105695 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729685 GSM8105696_r1 SRP491500 PRJNA1079999 SRS20558447 GSM8105696 GSM8105696 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729686 GSM8105697_r1 SRP491500 PRJNA1079999 SRS20558442 GSM8105697 GSM8105697 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729687 GSM8105698_r1 SRP491500 PRJNA1079999 SRS20558444 GSM8105698 GSM8105698 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729688 GSM8105699_r1 SRP491500 PRJNA1079999 SRS20558445 GSM8105699 GSM8105699 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729689 GSM8105700_r1 SRP491500 PRJNA1079999 SRS20558446 GSM8105700 GSM8105700 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729690 GSM8105701_r1 SRP491500 PRJNA1079999 SRS20558448 GSM8105701 GSM8105701 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729691 GSM8105702_r1 SRP491500 PRJNA1079999 SRS20558449 GSM8105702 GSM8105702 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729692 GSM8105703_r1 SRP491500 PRJNA1079999 SRS20558450 GSM8105703 GSM8105703 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729693 GSM8105704_r1 SRP491500 PRJNA1079999 SRS20558451 GSM8105704 GSM8105704 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729694 GSM8105705_r1 SRP491500 PRJNA1079999 SRS20558452 GSM8105705 GSM8105705 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729695 GSM8105706_r1 SRP491500 PRJNA1079999 SRS20558453 GSM8105706 GSM8105706 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729696 GSM8105707_r1 SRP491500 PRJNA1079999 SRS20558454 GSM8105707 GSM8105707 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729697 GSM8105708_r1 SRP491500 PRJNA1079999 SRS20558455 GSM8105708 GSM8105708 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729698 GSM8105709_r1 SRP491500 PRJNA1079999 SRS20558456 GSM8105709 GSM8105709 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729699 GSM8105710_r1 SRP491500 PRJNA1079999 SRS20558457 GSM8105710 GSM8105710 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729700 GSM8105711_r1 SRP491500 PRJNA1079999 SRS20558458 GSM8105711 GSM8105711 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729701 GSM8105712_r1 SRP491500 PRJNA1079999 SRS20558459 GSM8105712 GSM8105712 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729702 GSM8105713_r1 SRP491500 PRJNA1079999 SRS20558460 GSM8105713 GSM8105713 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729703 GSM8105714_r1 SRP491500 PRJNA1079999 SRS20558461 GSM8105714 GSM8105714 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729704 GSM8105715_r1 SRP491500 PRJNA1079999 SRS20558462 GSM8105715 GSM8105715 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729705 GSM8105716_r1 SRP491500 PRJNA1079999 SRS20558463 GSM8105716 GSM8105716 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729706 GSM8105717_r1 SRP491500 PRJNA1079999 SRS20558464 GSM8105717 GSM8105717 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729707 GSM8105718_r1 SRP491500 PRJNA1079999 SRS20558465 GSM8105718 GSM8105718 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729708 GSM8105719_r1 SRP491500 PRJNA1079999 SRS20558466 GSM8105719 GSM8105719 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729709 GSM8105720_r1 SRP491500 PRJNA1079999 SRS20558467 GSM8105720 GSM8105720 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729710 GSM8105721_r1 SRP491500 PRJNA1079999 SRS20558468 GSM8105721 GSM8105721 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729711 GSM8105722_r1 SRP491500 PRJNA1079999 SRS20558469 GSM8105722 GSM8105722 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729712 GSM8105723_r1 SRP491500 PRJNA1079999 SRS20558471 GSM8105723 GSM8105723 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729713 GSM8105724_r1 SRP491500 PRJNA1079999 SRS20558470 GSM8105724 GSM8105724 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729714 GSM8105725_r1 SRP491500 PRJNA1079999 SRS20558472 GSM8105725 GSM8105725 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000 SRX23729715 GSM8105726_r1 SRP491500 PRJNA1079999 SRS20558473 GSM8105726 GSM8105726 ATAC-seq GENOMIC other ~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed. Illumina NovaSeq 6000