SRP491834 PRJNA1080688 PN and anammox activated sludge sample In a two-stage PNA system sequentially treating synthetic wastewater and incineration leachate over 230 days, sludge samples were collected in triplicate on day 109 during the synthetic wastewater operational phase and day 232 during the incineration leachate operational phase from the PN and anammox stages.