SRX690997 GSM1495450 SRP046006 SRS693464 GSM1495450 ChIP-Seq GENOMIC ChIP 30E7 CD4+ T cells were fixed with 1% formaldehyde (25°C, 10 min) and stopped with 0.125M Glycine. Nuclei were sonicated using a Diagenode Bioruptor (Liege) to produce fragments of ~200 bp. LEDGF/p75-bound chromatin fragments were immunoprecipitated with antibody A300-848. Libraries were generated according to the Illumina TruSeq DNA Sample preparation guide. Briefly, the immunoprecipitated DNA was end-repaired, A-tailed and ligated to TruSeq adapters. After PCR amplification with 15 cycles and gel size selection of ~300bp fragments, libraries were captured and sequenced using the Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample GSM1495450 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1495450 gds 301495450 GEO Accession GSM1495450 SRX690998 GSM1495451 SRP046006 SRS693465 GSM1495451 ChIP-Seq GENOMIC ChIP 30E7 CD4+ T cells were fixed with 1% formaldehyde (25°C, 10 min) and stopped with 0.125M Glycine. Nuclei were sonicated using a Diagenode Bioruptor (Liege) to produce fragments of ~200 bp. LEDGF/p75-bound chromatin fragments were immunoprecipitated with antibody A300-848. Libraries were generated according to the Illumina TruSeq DNA Sample preparation guide. Briefly, the immunoprecipitated DNA was end-repaired, A-tailed and ligated to TruSeq adapters. After PCR amplification with 15 cycles and gel size selection of ~300bp fragments, libraries were captured and sequenced using the Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample GSM1495451 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1495451 gds 301495451 GEO Accession GSM1495451