SRX23769663 NL_P2_HiC SRP492105 bp0 Hi-C library preparation was performed using the Arima Hi-C Plus kit (Arima, USA) following the manufacturers protocol for large animal tissue, using the DpnII and HinfI enzymes. Up to 50 mg of fresh Cetoscarus ocellatus muscle tissue was flash frozen and pulverized using liquid nitrogen. A crosslinking buffer containing 1% formaldehyde was added to the pulverized tissue to crosslink DNA. We used 500 ng of DNA for Hi-C library preparation. The final library preparation for sequencing was performed using the KAPA Hyper Prep kit following the protocol detailed in the Arima Hi-C Plus kit. Sequencing was carried out on the Novaseq platform (Illumina, USA) using Novaseq S4 flow cell 2150 bp at Novogene (USA). SRS20595187 PF1 NL_P2_HiC Hi-C GENOMIC RANDOM Illumina NovaSeq 6000 SRX23769664 T506_TELL-Seq SRP492105 bp0 A Transposase Enzyme-Linked Long-read Sequencing (TELL-seq) library was constructed using a TELL-seq WGS Library Prep Kit (Universal Sequencing Technology). DNA extracted from Cetoscarus ocellatus was incubated with ~8 million TELL beads for barcoding according to the manufacturers protocol. Each TELL bead comprises 50,000 copies of at least one barcode sequence conjugated to its surface. Following barcoding, an eight-cycle amplification step was carried out in order to generate libraries for sequencing. TELL-seq data were generated by the Australian Genome Research Facility. SRS20595187 PF1 T506_TELL-Seq WGS GENOMIC RANDOM Illumina HiSeq 4000 SRX23769665 m64186e_210210_181112 SRP492105 bp0 Single-molecule real-time sequencing bell (SMRT Bell) library using DNA extracted from Cetoscarus ocellatus was prepared using a PacBio DNA Template Prep Kit 1.0 (Pacific Biosciences). The SMRT Bell-polymerase complex was constructed using a PacBio Binding Kit 2.0 (Pacific Biosciences), based on the manufacturers instructions. The library was loaded onto SMRT cells (Sequel SMRT Cell 1M v2, Pacific Biosciences) and sequenced using Sequel Sequencing Kit 2.1. SRS20595187 PF1 m64186e_210210_181112 WGS GENOMIC RANDOM Sequel