SRX23769590 GSM8115039_r1 SRP492102 PRJNA1081384 SRS20595128 GSM8115039 GSM8115039 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769591 GSM8115040_r1 SRP492102 PRJNA1081384 SRS20595130 GSM8115040 GSM8115040 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769592 GSM8115041_r1 SRP492102 PRJNA1081384 SRS20595134 GSM8115041 GSM8115041 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769593 GSM8115042_r1 SRP492102 PRJNA1081384 SRS20595500 GSM8115042 GSM8115042 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769594 GSM8115043_r1 SRP492102 PRJNA1081384 SRS20595135 GSM8115043 GSM8115043 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769595 GSM8115044_r1 SRP492102 PRJNA1081384 SRS20595133 GSM8115044 GSM8115044 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769596 GSM8115045_r1 SRP492102 PRJNA1081384 SRS20595639 GSM8115045 GSM8115045 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769597 GSM8115046_r1 SRP492102 PRJNA1081384 SRS20595501 GSM8115046 GSM8115046 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769598 GSM8115047_r1 SRP492102 PRJNA1081384 SRS20595136 GSM8115047 GSM8115047 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769599 GSM8115048_r1 SRP492102 PRJNA1081384 SRS20595138 GSM8115048 GSM8115048 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769600 GSM8115049_r1 SRP492102 PRJNA1081384 SRS20595137 GSM8115049 GSM8115049 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769601 GSM8115050_r1 SRP492102 PRJNA1081384 SRS20595503 GSM8115050 GSM8115050 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769602 GSM8115051_r1 SRP492102 PRJNA1081384 SRS20595139 GSM8115051 GSM8115051 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769603 GSM8115052_r1 SRP492102 PRJNA1081384 SRS20595140 GSM8115052 GSM8115052 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769604 GSM8115053_r1 SRP492102 PRJNA1081384 SRS20595637 GSM8115053 GSM8115053 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769605 GSM8115054_r1 SRP492102 PRJNA1081384 SRS20595502 GSM8115054 GSM8115054 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769606 GSM8115055_r1 SRP492102 PRJNA1081384 SRS20595141 GSM8115055 GSM8115055 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769607 GSM8115056_r1 SRP492102 PRJNA1081384 SRS20595143 GSM8115056 GSM8115056 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769608 GSM8115057_r1 SRP492102 PRJNA1081384 SRS20595142 GSM8115057 GSM8115057 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769609 GSM8115058_r1 SRP492102 PRJNA1081384 SRS20595144 GSM8115058 GSM8115058 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769610 GSM8115059_r1 SRP492102 PRJNA1081384 SRS20595145 GSM8115059 GSM8115059 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769611 GSM8115060_r1 SRP492102 PRJNA1081384 SRS20595147 GSM8115060 GSM8115060 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769612 GSM8115061_r1 SRP492102 PRJNA1081384 SRS20595504 GSM8115061 GSM8115061 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769613 GSM8115062_r1 SRP492102 PRJNA1081384 SRS20595150 GSM8115062 GSM8115062 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769614 GSM8115063_r1 SRP492102 PRJNA1081384 SRS20595146 GSM8115063 GSM8115063 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769615 GSM8115064_r1 SRP492102 PRJNA1081384 SRS20595148 GSM8115064 GSM8115064 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769616 GSM8115065_r1 SRP492102 PRJNA1081384 SRS20595149 GSM8115065 GSM8115065 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000 SRX23769617 GSM8115066_r1 SRP492102 PRJNA1081384 SRS20595505 GSM8115066 GSM8115066 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Nuclei were isolated from frozen human brain tumor and non-tumor tissues. Briefly, frozen human tissue samples were homogenized and lysed with Triton X-100 in RNase-free water for nuclei isolation. The isolated nuclei were purified, centrifuged, and resuspended in PBS with BSA and RNAse Inhibitor. The nuclei were diluted to 700 nuclei/ul and loaded to 10x Genomics Chromium Controller to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 3' single nucleus gene expression libraries (Next GEM v3.1, dual index) were constructed using the 10x Genomics Chromium system. Amplified cDNAs and the libraries were measured by Qubit dsDNA HS assay and quality assessed by BioAnalyzer. Each library was sequenced on Illumina NovaSeq 6000 (PE150). Reads were subsequently processed using 10x Genomics Cell Ranger analytical pipeline and human GRCh38 reference genome. Illumina NovaSeq 6000