SRX23759441 GSM8114445_r1 SRP491971 PRJNA1081308 SRS20585054 GSM8114445 GSM8114445 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Cells of the limb muscles were isolated mechanically and enzymaticaly using trypsin centrifuged and resuspended with BioAmf. Using a wide-bore pipette tip cells were thoroughly mixed to get single cells, centrifuged and resuspended with 1X PBS with 0.04% BSA. Two RNA single cell libraries were prepared according to 10x manufacture protocol (Chromium Next GEM Single Cell 3' Library & Gel Bead Kit v3.1, PN-1000121) using 20,000 input cells per sample (10,000 target cells per sample). Illumina NextSeq2000 (Illumina, cat no. 20040559) NextSeq 2000 SRX23759442 GSM8114446_r1 SRP491971 PRJNA1081308 SRS20585053 GSM8114446 GSM8114446 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Cells of the limb muscles were isolated mechanically and enzymaticaly using trypsin centrifuged and resuspended with BioAmf. Using a wide-bore pipette tip cells were thoroughly mixed to get single cells, centrifuged and resuspended with 1X PBS with 0.04% BSA. Two RNA single cell libraries were prepared according to 10x manufacture protocol (Chromium Next GEM Single Cell 3' Library & Gel Bead Kit v3.1, PN-1000121) using 20,000 input cells per sample (10,000 target cells per sample). Illumina NextSeq2000 (Illumina, cat no. 20040559) NextSeq 2000