SRX23774876 Ancestral_Recipient_CFP SRP462258 PRJNA1019258 The library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, a clone of E. coli was grown in LB and DNA was extracted for whole genome sequencing in a NextSeq2000 SRS20600386 Ancestral_Recipient_CFP Ancestral_Recipient_CFP WGS GENOMIC RANDOM NextSeq 2000 SRX23774877 Ancestral_Recipient_YFP SRP462258 PRJNA1019258 The library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, a clone of E. coli was grown in LB and DNA was extracted for whole genome sequencing in a NextSeq2000 SRS20600387 Ancestral_Recipient_YFP Ancestral_Recipient_YFP WGS GENOMIC RANDOM NextSeq 2000 SRX23774878 Ancestral_Donor_sfGFP SRP462258 PRJNA1019258 The library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, a clone of E. coli was grown in LB and DNA was extracted for whole genome sequencing in a NextSeq2000 SRS20600388 Ancestral_Donor_sfGFP Ancestral_Donor_sfGFP WGS GENOMIC RANDOM NextSeq 500 SRX23774879 Ancestral_Donor_mCherry SRP462258 PRJNA1019258 The library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, a clone of E. coli was grown in LB and DNA was extracted for whole genome sequencing in a NextSeq2000 SRS20600389 Ancestral_Donor_mCherry Ancestral_Donor_mCherry WGS GENOMIC RANDOM NextSeq 500