SRX23797615 Blank1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620654 Blank1 Blank1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797616 Blank2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620656 Blank2 Blank2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797617 T0P3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620655 Sample_7 T0P3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797618 T0P4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620657 Sample_8 T0P4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797619 T1F11 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620658 Sample_9 T1F11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797620 T1F12 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620659 Sample_10 T1F12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797621 T1F13 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620660 Sample_11 T1F13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797622 T1F14 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620661 Sample_12 T1F14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797623 T1F15 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620662 Sample_13 T1F15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797624 T1P11 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620663 Sample_14 T1P11 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797625 T1P12 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620665 Sample_15 T1P12 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797626 T1P13 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620664 Sample_16 T1P13 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797627 Negative SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620666 Negative Negative AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797628 T1P14 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620667 Sample_17 T1P14 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797629 T1P15 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620668 Sample_18 T1P15 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797630 T2F10 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620669 Sample_19 T2F10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797631 T2F6 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620670 Sample_20 T2F6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797632 T2F7 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620671 Sample_21 T2F7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797633 T2F8 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620672 Sample_22 T2F8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797634 T2F9 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620674 Sample_23 T2F9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797635 T2P10 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620673 Sample_24 T2P10 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797636 T2P6 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620675 Sample_25 T2P6 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797637 T2P7 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620676 Sample_26 T2P7 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797638 Positive SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620677 Positive Positive AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797639 T2P8 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620678 Sample_27 T2P8 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797640 T2P9 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620679 Sample_28 T2P9 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797641 T3F1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620680 Sample_29 T3F1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797642 T3F2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620681 Sample_30 T3F2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797643 T3F3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620682 Sample_31 T3F3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797644 T3F4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620683 Sample_32 T3F4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797645 T3F5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620684 Sample_33 T3F5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797646 T3P1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620685 Sample_34 T3P1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797647 TFE1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620686 Sample_49 TFE1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797648 TFE2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620687 Sample_50 TFE2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797649 TFE3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620688 Sample_51 TFE3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797650 TFE4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620689 Sample_52 TFE4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797651 TFE5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620690 Sample_53 TFE5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797652 TOF2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620691 Sample_54 TOF2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797653 TOP5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620692 Sample_55 TOP5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797654 T0F4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620693 Sample_3 T0F4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797655 T0F5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620694 Sample_4 T0F5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797656 T0P1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620695 Sample_5 T0P1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797657 T0P2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620696 Sample_6 T0P2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797658 T3P2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620698 Sample_35 T3P2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797659 T3P3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620697 Sample_36 T3P3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797660 T0F1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620700 Sample_1 T0F1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797661 T3P4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620699 Sample_37 T3P4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797662 T3P5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620701 Sample_38 T3P5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797663 T4F1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620702 Sample_39 T4F1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797664 T4F2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620703 Sample_40 T4F2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797665 T4F3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620704 Sample_41 T4F3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797666 T4F4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620705 Sample_42 T4F4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797667 T4F5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620706 Sample_43 T4F5 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797668 T4P1 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620707 Sample_44 T4P1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797669 T4P2 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620708 Sample_45 T4P2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797670 T4P3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620709 Sample_46 T4P3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797671 T0F3 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620711 Sample_2 T0F3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797672 T4P4 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620710 Sample_47 T4P4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX23797673 T4P5 SRP492615 bp0 The V3 and V4 regions of the 16s rRNA gene were amplified from the extracted DNA by Polymerase Chain Reaction (PCR) using the 341F and 785R primer pair with Illumina MiSeq adapter overhang sequences added to the gene-specific nucleotide sequences. The sequences of the primers were: 341F = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, 785R = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. The reaction was prepared with the KAPA HiFi HotStart ReadyMix (Roche, Switzerland), with 5 M of each primer, 12.5 ng of DNA and 12.5 L of the Mix, diluted with DNAse-free water to a volume of 25 L. The temperature profile for amplification was as follows: one cycle of five minutes at 92 oC, followed by 30 cycles of 30 seconds at 92 oC, 30 seconds at 62 oC and 30 seconds at 72 oC, followed by one cycle at 72 oC. For each sample, reactions were done in triplicate and then combined in a single pool. All sequences were checked by agarose gel electrophoresis for correct amplification before pooling. The resulting PCR product was sent to Macrogen (Seoul, South Korea) for DNA sequencing using the MiSeq sequencing platform. SRS20620712 Sample_48 T4P5 AMPLICON METAGENOMIC PCR Illumina MiSeq