SRX23809087 GSM8122007_r1 SRP492783 PRJNA1082649 SRS20631976 GSM8122007 GSM8122007 RNA-Seq TRANSCRIPTOMIC cDNA Firstly, total RNA was isolated from cardiac tissue by TRIzol Reagent and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then RNA-seq library were preparated, after PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system, clustering and sequencing on an Illumina Novaseq platform. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Illumina NovaSeq 6000 SRX23809088 GSM8122008_r1 SRP492783 PRJNA1082649 SRS20631977 GSM8122008 GSM8122008 RNA-Seq TRANSCRIPTOMIC cDNA Firstly, total RNA was isolated from cardiac tissue by TRIzol Reagent and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then RNA-seq library were preparated, after PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system, clustering and sequencing on an Illumina Novaseq platform. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Illumina NovaSeq 6000 SRX23809089 GSM8122009_r1 SRP492783 PRJNA1082649 SRS20631978 GSM8122009 GSM8122009 RNA-Seq TRANSCRIPTOMIC cDNA Firstly, total RNA was isolated from cardiac tissue by TRIzol Reagent and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then RNA-seq library were preparated, after PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system, clustering and sequencing on an Illumina Novaseq platform. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Illumina NovaSeq 6000 SRX23809090 GSM8122010_r1 SRP492783 PRJNA1082649 SRS20631979 GSM8122010 GSM8122010 RNA-Seq TRANSCRIPTOMIC cDNA Firstly, total RNA was isolated from cardiac tissue by TRIzol Reagent and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then RNA-seq library were preparated, after PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system, clustering and sequencing on an Illumina Novaseq platform. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Illumina NovaSeq 6000 SRX23809091 GSM8122011_r1 SRP492783 PRJNA1082649 SRS20631980 GSM8122011 GSM8122011 RNA-Seq TRANSCRIPTOMIC cDNA Firstly, total RNA was isolated from cardiac tissue by TRIzol Reagent and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then RNA-seq library were preparated, after PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system, clustering and sequencing on an Illumina Novaseq platform. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Illumina NovaSeq 6000 SRX23809092 GSM8122012_r1 SRP492783 PRJNA1082649 SRS20631981 GSM8122012 GSM8122012 RNA-Seq TRANSCRIPTOMIC cDNA Firstly, total RNA was isolated from cardiac tissue by TRIzol Reagent and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then RNA-seq library were preparated, after PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system, clustering and sequencing on an Illumina Novaseq platform. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Illumina NovaSeq 6000