SRX23809454 GSM8122156_r1 SRP492817 PRJNA1082719 SRS20632335 GSM8122156 GSM8122156 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809455 GSM8122157_r1 SRP492817 PRJNA1082719 SRS20632336 GSM8122157 GSM8122157 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809456 GSM8122158_r1 SRP492817 PRJNA1082719 SRS20632337 GSM8122158 GSM8122158 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809457 GSM8122159_r1 SRP492817 PRJNA1082719 SRS20632338 GSM8122159 GSM8122159 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809458 GSM8122160_r1 SRP492817 PRJNA1082719 SRS20632339 GSM8122160 GSM8122160 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809459 GSM8122161_r1 SRP492817 PRJNA1082719 SRS20632340 GSM8122161 GSM8122161 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809460 GSM8122162_r1 SRP492817 PRJNA1082719 SRS20632341 GSM8122162 GSM8122162 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809461 GSM8122163_r1 SRP492817 PRJNA1082719 SRS20632342 GSM8122163 GSM8122163 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809462 GSM8122164_r1 SRP492817 PRJNA1082719 SRS20632343 GSM8122164 GSM8122164 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809463 GSM8122165_r1 SRP492817 PRJNA1082719 SRS20632344 GSM8122165 GSM8122165 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809464 GSM8122166_r1 SRP492817 PRJNA1082719 SRS20632345 GSM8122166 GSM8122166 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809465 GSM8122167_r1 SRP492817 PRJNA1082719 SRS20632346 GSM8122167 GSM8122167 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809466 GSM8122168_r1 SRP492817 PRJNA1082719 SRS20632347 GSM8122168 GSM8122168 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809467 GSM8122169_r1 SRP492817 PRJNA1082719 SRS20632348 GSM8122169 GSM8122169 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000 SRX23809468 GSM8122170_r1 SRP492817 PRJNA1082719 SRS20632350 GSM8122170 GSM8122170 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from cells 24hrs after seededing at 70% confluence. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Illumina NovaSeq 6000