SRX23809317 GSM8122103_r1 SRP492795 PRJNA1082655 SRS20632203 GSM8122103 GSM8122103 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809318 GSM8122104_r1 SRP492795 PRJNA1082655 SRS20632202 GSM8122104 GSM8122104 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809319 GSM8122105_r1 SRP492795 PRJNA1082655 SRS20632206 GSM8122105 GSM8122105 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809320 GSM8122106_r1 SRP492795 PRJNA1082655 SRS20632204 GSM8122106 GSM8122106 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809321 GSM8122107_r1 SRP492795 PRJNA1082655 SRS20632205 GSM8122107 GSM8122107 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809322 GSM8122108_r1 SRP492795 PRJNA1082655 SRS20632207 GSM8122108 GSM8122108 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809323 GSM8122109_r1 SRP492795 PRJNA1082655 SRS20632208 GSM8122109 GSM8122109 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500 SRX23809324 GSM8122110_r1 SRP492795 PRJNA1082655 SRS20632209 GSM8122110 GSM8122110 OTHER TRANSCRIPTOMIC other Cells were lysed and RNA-Protein-complexes were immunoprecipitated with GFP-Trap magnetic agarose beads (GFP Nanobody-coupled beads) and subjected to on-bead RnaseI treatment. After stringent washing, RNA was dephosphorylated and ligated to a 3' linker. Protein-RNA complexes were resolved by denaturing gel electrophoresis and transfered to nitrocellulose membrane. A membrane region above the expected size of the protein was cut and RNA was released by Proteinase K treatment. RNA was reverse transcribed using reverse primer and a second adapter containing a barcode was ligated to the cDNAs. The cDNA was pre-amplified for 6 cycles using short Solexa P3 and P7 primers. After a size selection on beads a second PCR amplification was performed with the normal P3 and P7 primers to generate the final iCLIP2 libraries. NextSeq 500