SRX23811285 GSM8122831_r1 SRP492861 PRJNA1082733 SRS20634079 GSM8122831 GSM8122831 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811286 GSM8122832_r1 SRP492861 PRJNA1082733 SRS20634080 GSM8122832 GSM8122832 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811287 GSM8122833_r1 SRP492861 PRJNA1082733 SRS20634081 GSM8122833 GSM8122833 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811288 GSM8122834_r1 SRP492861 PRJNA1082733 SRS20634082 GSM8122834 GSM8122834 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811289 GSM8122835_r1 SRP492861 PRJNA1082733 SRS20634083 GSM8122835 GSM8122835 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811290 GSM8122836_r1 SRP492861 PRJNA1082733 SRS20634084 GSM8122836 GSM8122836 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811291 GSM8122837_r1 SRP492861 PRJNA1082733 SRS20634085 GSM8122837 GSM8122837 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811292 GSM8122838_r1 SRP492861 PRJNA1082733 SRS20634087 GSM8122838 GSM8122838 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811293 GSM8122839_r1 SRP492861 PRJNA1082733 SRS20634086 GSM8122839 GSM8122839 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811294 GSM8122840_r1 SRP492861 PRJNA1082733 SRS20634088 GSM8122840 GSM8122840 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811295 GSM8122841_r1 SRP492861 PRJNA1082733 SRS20634089 GSM8122841 GSM8122841 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811296 GSM8122842_r1 SRP492861 PRJNA1082733 SRS20634090 GSM8122842 GSM8122842 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811297 GSM8122843_r1 SRP492861 PRJNA1082733 SRS20634091 GSM8122843 GSM8122843 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811298 GSM8122844_r1 SRP492861 PRJNA1082733 SRS20634092 GSM8122844 GSM8122844 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811299 GSM8122845_r1 SRP492861 PRJNA1082733 SRS20634093 GSM8122845 GSM8122845 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000 SRX23811300 GSM8122846_r1 SRP492861 PRJNA1082733 SRS20634094 GSM8122846 GSM8122846 RNA-Seq TRANSCRIPTOMIC cDNA Following the differentiation, enteroids were placed in Organoid Harvesting Solution (3700-100-01; Cultrex) for 1 hr at 4˚C. Enteroids were washed with PBS and centrifuged. Enteroid pellets were immersed in TRIzol reagent (15596026; Invitrogen) supplemented with glycogen (G1767; Sigma Aldrich) Libraries were prepared by Novogene Corporation Inc. (Sacramento, CA). mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer. Then first strand cDNA is synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. Double-stranded cDNA is purified using AMPure XP beads. Remaining overhangs of the purified doublestranded cDNA are converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare for hybridization(1). In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments are purified with AMPure XP system (Beckman Coulter, Beverly, USA). Finally, the final library is gotten by PCR amplification and purification of PCR products by AMPure XP beads. Illumina NovaSeq 6000